棉酚降解酶对棉籽粕酶解脱毒的条件参数优化

doi:10.6048/j.issn.1001-4330.2019.06.015

新疆农业科学 ›› 2019, Vol. 56 ›› Issue (6): 1122-1126.DOI: 10.6048/j.issn.1001-4330.2019.06.015

• 土壤肥料·贮藏加工·植物保护·畜牧水产 • 上一篇下一篇

棉酚降解酶对棉籽粕酶解脱毒的条件参数优化

杨文婷, 陈程, 张文举

石河子大学动物科技学院,新疆石河子 832000

出版日期:2019-06-20 发布日期:2019-08-08
通信作者: 张文举(1966-),男,陕西人,教授,研究方向为饲料资源的开发与利用,(E-mail)zhangwj1022@ sina.com
作者简介:杨文婷(1994-),河南人,硕士研究生,研究方向为饲料资源的开发与利用,(E-mail)891633254@qq.com
基金资助:
国家自然科学基金(31860660)

Optimization of the Conditions for DetoxificatioofCottonseed Meal by Gossypol Degradation Enzyme

YANG Wen-ting, CHEN Cheng, ZHANG Wen-ju

College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 83200, China

Online:2019-06-20 Published:2019-08-08
Correspondence author: ZHANG Wen-ju(1966-),male,native place: ShanXi. Professor, research field:Development and utilization of feed resources,(E-mail) zhangwj1022@ sina.com
Supported by:
Supported by the National Natural Science Foundation of China(31860660)

摘要/Abstract

摘要： 【目的】优化棉酚降解酶对棉籽粕酶解脱毒的条件参数。【方法】采用4因子4水平的正交试验设计,以游离棉酚的含量为指标筛选出棉籽粕的最佳酶解条件参数。【结果】棉酚降解酶对棉籽粕酶解脱毒的最佳条件参数为温度35℃、时间1.5 h、添加量3%、底物水分35%。【结论】筛选出的酶解脱毒参数具有显著的效果,在最优酶解参数条件下,通过试验验证,棉籽粕中游离棉酚的含量由341.02 mg/kg降至37.01 mg/kg,游离棉酚脱毒率可达89.15%,可以应用于对棉籽粕游离棉酚的酶解脱毒。

关键词: 棉酚降解酶; 游离棉酚; 酶解脱毒 ; 正交设计

Abstract: 【Objective】 To optimize the condition parameters of enzymatic hydrolysis and detoxification of cottonseed meal by gossypol degrading enzyme. 【Method】 Through the orthogonal design of 4 factors and 4 levels, the optimum conditions of hydrolysis of cottonseed meal were selected with the content of free gossypol as the index. 【Result】The optimum conditions for the detoxification of cottonseed meal by gossypol-degrading enzyme were as follows: Temperature 35℃, time 1.5 h, inoculation amount 3%, the substrate moisture 35%. 【Conclusion】The selected enzymatic detoxification parameters have significant effects. Under the conditions of optimal hydrolysis parameters, it was verified by experiments that the content of free gossypol in cottonseed meal decreased from 341.02 mg/kg to 37.01 mg/kg, and the degradation rate of free gossypol was 89.15%, which could be applied to the enzyme-catalyzed detoxification of free gossypol in cottonseed meal.

Key words: gossypol degradation enzyme; free gossypol; enzyme-catalyzed detoxification; orthogonal experimental design

中图分类号:

S188

杨文婷, 陈程, 张文举. 棉酚降解酶对棉籽粕酶解脱毒的条件参数优化[J]. 新疆农业科学, 2019, 56(6): 1122-1126.

YANG Wen-ting, CHEN Cheng, ZHANG Wen-ju. Optimization of the Conditions for DetoxificatioofCottonseed Meal by Gossypol Degradation Enzyme[J]. Xinjiang Agricultural Sciences, 2019, 56(6): 1122-1126.

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棉酚降解酶对棉籽粕酶解脱毒的条件参数优化

Optimization of the Conditions for DetoxificatioofCottonseed Meal by Gossypol Degradation Enzyme

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