不同油气储藏区微生物代谢活性及多样性响应

doi:10.6048/j.issn.1001-4330.2018.10.020

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (10): 1927-1935.DOI: 10.6048/j.issn.1001-4330.2018.10.020

不同油气储藏区微生物代谢活性及多样性响应

楚敏,林青,张志东,高雁,史应武,杨红梅,霍向东,顾美英,曾军,李玉国,欧提库尔,张涛

新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091

发布日期:2019-01-24
通信作者: 张涛(1979-),男,新疆乌鲁木齐人,副研究员,研究方向为微生物学,(E-mail)25439517@qq.com
作者简介:楚敏(1977-),女,新疆乌鲁木齐人,副研究员,研究方向为微生物学与分子生物学,(E-mail)chuliu2002@163.com
基金资助:
新疆维吾尔自治区自然科学基金“油气微生物勘探技术中微生物异常检测方法创新研究”(31460027)

Microbial Metabolic Activity and Diversity Response in Different Oil and Gas Storage Areas

CHU Min, LING Qing, ZHANG Zhi-dong, GAO Yan, SHI Yin-wu, YANG Hong-mei, HUO XIANG-dong, GU Mei-ying, ZENG Jun, LI Yu-guo, Otkur, ZHANG Tao

Research Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Published:2019-01-24
Correspondence author: ZHANG Tao(1977-), male, Urumqi,Xinjiang,associate researcher, research field:microbiology, (E-mail)25439517@qq.com

摘要/Abstract

摘要： 【目的】 研究土壤微生物群落代谢活性及多样性对地下油气储藏情况的响应。【方法】 采用Biolog-ECO微平板技术,研究焉耆境内不同油气储藏地区,地下80~100 cm土壤样品微生物的群落代谢活性及多样性。【结果】 微生物代谢活性总体呈现利于藏油气区域微生物活性>不利于或难于评价藏油气区域>无藏油气区域的规律;在利于藏油气和不利于或难评价藏油气区域样品中,氨基酸和多聚物利用微生物在AWCD的贡献上均高于对照区域,而羧酸、胺类均低于对照区域。主成分分析表明,三个不同藏油气区域微生物群落分布特征有着明显差异,PCA 1、PCA 2中权重最大的基质为D-甘露醇和D-木糖,分别为载荷值0.97和0.87。【结论】 新疆焉耆三个区域,地下土壤微生物群落明显受油气分布影响。Biolog -ECO微平板技术可能作为油气储藏初步勘测的一种手段。

关键词: Biolog-ECO; 油气储藏; 微生物代谢多样性; 群落代谢活性

Abstract: 【Objective】 To study the response of soil microbial community metabolic activity and diversity to underground oil and gas storage.【Method】 The metabolic activity and diversity of microbial community in 80-100 cm soil samples from different oil and gas storage areas in Yanqi were studied by using Biolog-ECO microplate technique.【Result】 In the three areas of Yanqi, Xinjiang that the study analyzed, soil microbial communities were obviously affected by oil and gas distribution. The general rules of microbial metabolic activity were as follows: the microbial activity of favorable reservoir oil and gas region > unfavorable or difficult to evaluate reservoir oil and gas area > no reservoir oil and gas area; The contribution of amino acid and polymer utilization microbes to AWCD in the samples that were favorable to reservoir oil and gas or difficult to evaluate was higher than that in the control area, while carboxyl and amines were lower than those in the control area. Results of principal component analysis showed that the distribution characteristics of microbial communities in three different reservoirs were obviously different, and the most powerful matrixes was the D-mannitol and D-xylose in PCA 1 and PCA 2, whose respective load values were 0.97 and 0.87.【Conclusion】 Biolog-ECO microplate technology can be used as a means for preliminary exploration of oil and gas reservoirs.

Key words: biolog-ECO; oil and gas storage; microbial metabolic diversity; metabolic activity of community

楚敏,林青,张志东,高雁,史应武,杨红梅,霍向东,顾美英,曾军,李玉国,欧提库尔,张涛. 不同油气储藏区微生物代谢活性及多样性响应[J]. 新疆农业科学, 2018, 55(10): 1927-1935.

CHU Min, LING Qing, ZHANG Zhi-dong, GAO Yan, SHI Yin-wu, YANG Hong-mei, HUO XIANG-dong, GU Mei-ying, ZENG Jun, LI Yu-guo, Otkur, ZHANG Tao. Microbial Metabolic Activity and Diversity Response in Different Oil and Gas Storage Areas[J]. Xinjiang Agricultural Sciences, 2018, 55(10): 1927-1935.

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不同油气储藏区微生物代谢活性及多样性响应

Microbial Metabolic Activity and Diversity Response in Different Oil and Gas Storage Areas

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