]*>","")" /> Optimization of Spore Production Medium and Conditions of Antagonistic Strain S13 of Bacillus amyloliauefaciens against Fusarium wilt Tomato
Xinjiang Agricultural Sciences

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Optimization of Spore Production Medium and Conditions of Antagonistic Strain S13 of Bacillus amyloliauefaciens against Fusarium wilt Tomato

HOU Min;ZHAN Fa-qiang;ZHANG Hui-tao;HOU Xin-qiang;YANG Rong;LONG Xuan-qi   

  • Received:2014-07-25 Revised:2014-07-25 Online:2014-07-25 Published:2014-07-25

番茄枯萎病拮抗细菌S13产芽孢发酵培养基及发酵条件优化

侯敏;詹发强;张慧涛;侯新强;杨蓉;龙宣杞   

  1. 新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐,830091





摘要： [目的]提高番茄枯萎病菌拮抗细菌解淀粉芽孢杆菌(Bacillus amyloliauefaciens)S13的芽孢数量和芽孢产率.[方法]采用单因素和正交试验,研究不同的氮源、碳源、pH、温度、培养时间对解淀粉芽孢杆菌形成芽孢的影响.[结果]确定解淀粉芽孢杆菌产芽孢培养基的最佳组分为(g/L):玉米粉0.4,酵母膏0.1,玉米浆1.25,KH2P040.1,K2 HP04 0.1,MgS04.7H20 0.05.对发酵条件进行了研究,产芽孢最适pH为7.0,最适温度为35℃,最适时间为26 h.[结论]通过优化培养基组分和培养基条件,1 000 L罐发酵液中芽孢数达到4.9×109cfu/mL,芽孢产率为90;,为进一步该菌株在复合生物肥中的应用打下基础.