Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (7): 1709-1719.DOI: 10.6048/j.issn.1001-4330.2025.07.016

• Cotton column • Previous Articles     Next Articles

Research on the optimal combination of plant growth regulators based on quadratic universal rotation combination

YANG Liu1,2(), TANG Guangmu1,2(), LIU Jiao2, ZHU Jie1,2, GUO Keyu1,2, ZHANG Yunshu1,2, MA Haigang2, XU Wanli1,2()   

  1. 1. College of Resources and Environment, Xinjiang Agricultural University, Urumqi 830052, China
    2. Key Laboratory of Saline-alkali Soil Improvement and Utilization (Saline-Alkali Land in Arid and Semi-Arid Regions), Ministry of Agriculture and Rural Affairs/Research Institute of Soil, Insitute of Agricultural Resources and Environment, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2024-12-08 Online:2025-07-20 Published:2025-09-05
  • Correspondence author: TANG Guangmu, XU Wanli
  • Supported by:
    Key R&D Program of Xinjiang Uygur Autonomous Region "Research on Green Key Technologies of Agricultural and Forestry Waste Pyrolysis Products"(2022B02013-3);Graduate Research Innovation Project of Xinjiang Agricultural University(XJAUGRI2024038)

基于二次通用旋转组合的植物生长调节剂最优组合

杨柳1,2(), 唐光木1,2(), 刘娇2, 朱杰1,2, 郭珂妤1,2, 张云舒1,2, 马海刚2, 徐万里1,2()   

  1. 1.新疆农业大学资源与环境学院, 乌鲁木齐 830052
    2.新疆维吾尔自治区农业科学院农业资源与环境研究所/农业农村部盐碱地改良与利用(干旱半干旱区盐碱地)重点实验室, 乌鲁木齐 830091
  • 通讯作者: 唐光木,徐万里
  • 作者简介:杨柳(1998-),女,内蒙古呼伦贝尔人,硕士研究生,研究方向为土壤改良与利用,(E-mail)59959383@qq.com
  • 基金资助:
    新疆维吾尔自治区重点研发计划项目“农林废弃物热解产物绿色关键技术研究”(2022B02013-3);新疆农业大学研究生科研创新项目(XJAUGRI2024038)

Abstract:

【Objective】 In order to investigate the effects of compound sodium dinitrophenol (CSN), plant polysaccharides (DSK), succinyl (D2), and compound nanooxygen (FN-6) on the stress resistance of cotton plants, the optimal combination ratio is going to be sought. 【Methods】 This study used a quadratic universal rotation combination design to determine the content of plant peroxidase (POD), catalase (CAT), superoxide dismutase (SDO), malondialdehyde (MDA), and indoleacetic acid (IAA). Design Expert 13 was used to establish a functional model for it. F-test was used to analyze the significance of each regression model and regression coefficient. Afterwards, the model would be used to analyze the effect of PGR application on plant enzyme activity and endogenous IAA content and entropy weight method was used to select plant POD activity as a representative indicator to simulate and screen the optimal combination plan for its regression model. Then the optimal application ratio was obtained. At the same time, the optimization results of representative indicators were verified through four comprehensive evaluation methods: TOPSIS method (Ci), entropy method (Si), factor analysis (ε j), and rank sum ratio comprehensive evaluation method (RSR). The consistent results showed that the best application effect was achieved at the zero level, and the application amount could affect plant enzyme activity and endogenous IAA content. 【Results】 With the increase of PGR application, the activities of POD, SOD, CAT and endogenous IAA in plants showed a trend of first increasing and then decreasing, while the MDA content showed a trend of first decreasing and then increasing. The impact of various factors on plant POD activity was as follows: DSK>FN-6>CSN>D2, the impact on plant CAT was as follows: FN-6>CSN>DSK>D2, and the impact on plant SOD, MDA, and endogenous IAA content was as follows: CSN>DSK>FN-6>D2. 【Conclusion】 Through experimental simulation, the optimal application plan is CSN: 446.04-448.73 mg/hm2 FN-6:526.28-528.96 mg/hm2, DSK:526.28-528.96 mg/hm2, D2:446.04-448.73 mg/hm2.

Key words: secondary universal rotation combination design; plant growth regulators; plant enzyme activity; seeking optimization

摘要:

【目的】探究复合双硝基酚钠(CSN)、植多苷(DSK)、丁双酰(D2)及复合奈氧(FN-6)对棉花植株抗逆能力的影响,寻求最佳配施比例。【方法】采用二次通用旋转组合设计,通过测定植株过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SDO)、丙二醛(MDA)及吲哚乙酸(IAA)含量,使用Design-Expert 13为其建立函数模型,F检验,分析各回归模型及各项回归系数的显著性,利用模型分析PGR施量对植株酶活性及内源IAA含量的影响,使用熵权法选取植株POD活性为代表性指示指标对其回归模型进行模拟与筛选优化组合方案,得到最优施用比例。同时,通过TOPSIS法(Ci)、熵值法(Si)、因子分析(εj)、秩和比综合评价法(RSR)四种综合评价方法对代表性指标寻优结果进行验证,结果一致均为处在零水平施用效果最佳,施量可影响植株酶活性及内源IAA含量。【结果】随着PGR施量的增加植株POD、SOD、CAT活性与内源IAA含量呈先增加后降低趋势,MDA含量呈先下降后增加趋势。各因素对植株POD活性的影响大小为DSK>FN-6>CSN>D2,对植株CAT的影响大小为FN-6>CSN>DSK>D2,对植株SOD、MDA及内源IAA含量的影响大小为均CSN>DSK>FN-6>D2。【结论】最佳配施方案为CSN:446.04~448.73 mg/hm2、FN-6:526.28~528.96 mg/hm2、DSK:526.28~528.96 mg/hm2、D2:446.04~448.73 mg/hm2

关键词: 二次通用旋转组合设计, 植物生长调节剂, 植株酶活, 寻优

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