Study on the mechanism of different forms of selenium on antioxidant ability and selenium metabolism of Hui jujube

doi:10.6048/j.issn.1001-4330.2025.05.013

Xinjiang Agricultural Sciences ›› 2025, Vol. 62 ›› Issue (5): 1159-1169.DOI: 10.6048/j.issn.1001-4330.2025.05.013

• Horticultural Special Local Products·Agricultural Product Processing Engineering • Previous Articles Next Articles

Study on the mechanism of different forms of selenium on antioxidant ability and selenium metabolism of Hui jujube

YUAN Yuting¹(), LIU Defen¹, SUN Wanjin³, JIA Wenzhao⁴, MA Shengjun¹(), KANG Lu²()

1. College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Agricultural Quality Standards and Testing Technology, Xinjiang Uyghur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China
3. Social Affairs Service Center of the Eighth Regiment of the First Division of Xinjiang Production and Construction Corps, Aral Xinjiang 843300, China
4. Agricultural Development Service Center of the Eighth Regiment of the First Division of Xinjiang Production and Construction Corps, Aral Xinjiang 843300, China

Received:2024-10-14 Online:2025-05-20 Published:2025-07-09
Correspondence author: MA Shengjun, KANG Lu
Supported by:
Basic Business Funds for Public Welfare Research Institutes in Xinjiang Uygur Autonomous Region(KY2024026);Xinjiang Production and construction Corps Science and Technology Plan Project(KY2024JBGS02)

不同形态硒调控灰枣抗氧化能力和硒代谢的机理分析

袁玉婷¹(), 刘德粉¹, 孙万金³, 贾文昭⁴, 马生军¹(), 康露²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆维吾尔自治区农业科学院农业质量标准与检测技术研究所,乌鲁木齐 830091
3.新疆生产建设兵团第一师八团社会事务服务中心,新疆阿拉尔 843300
4.新疆生产建设兵团第一师八团农业发展服务中心,新疆阿拉尔 843300

通讯作者: 马生军,康露
作者简介:袁玉婷(1999-),女,新疆喀什人,硕士研究生,研究方向为食品加工与安全,(E-mail)1486361352@qq.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2024026);师市科技计划项目(KY2024JBGS02)

Abstract

Abstract:

【Objective】 To study the effects of sodium selenite, nano-selenium and organic selenium on the appearance, antioxidant capacity and selenium metabolism of Hui jujube in the hope of providing theoretical basis for analyzing the accumulation mechanism of different selenium forms in plants. 【Methods】 Three treatments, sodium selenite, nano-selenium and organic selenium, were set up to determine the appearance of leaves and fruits, antioxidant enzyme activity and gene expression levels, selenoamino acids and gene expression levels related to selenium metabolism before and after treatment. 【Results】 After the application of nano-selenium, sodium selenite and organic selenium, the appearance morphology, antioxidant enzyme activity and gene expression, selenoamino acid content and selenium metabolization-related gene expression levels of Hui jujube were significantly increased, among which the hardness of Hui jujube increased by 14.40%-19.59%, soluble solid increased by 23.77%-37.43%. Glutathione peroxidase (GSH-Px) and gene expression were increased by 10.23%-110.41% and 8.18%-123.91%, respectively, total selenium content was increased by 3.09-34.14 times, selenometine (SeMet) was increased by 100.00%-346.15%. Cysteine desulphurase (CysD) gene expression increased by 18.13%-195.43%. 【Conclusion】 After nano-selenium, sodium selenite and organic selenium are transformed into selenoamino acids after spraying, different forms of selenium have effects on the appearance, antioxidant capacity and selenoamino acids of Hui jujube. In conclusion, organic selenium treatment is superior to sodium selenite and nano-selenium treatment.

Key words: Hui jujube; antioxidant capacity; selenium metabolism

摘要：

【目的】研究亚硒酸钠、纳米硒和有机硒3种不同形态硒对灰枣外观形态、抗氧化能力及硒代谢的影响,为分析不同形态硒在植物体内的积累机制提供理论依据。【方法】以灰枣为研究对象,设置亚硒酸钠、纳米硒和有机硒共3个处理,测定处理前后灰枣叶片和果实的外观形态,抗氧化酶活性及基因表达量,硒代氨基酸及硒代谢相关基因表达水平。【结果】施用纳米硒、亚硒酸钠和有机硒后,灰枣的外观形态,抗氧化酶活性及基因表达量,硒代氨基酸含量及硒代谢相关基因表达水平均显著增加,其中灰枣硬度增长14.40%~19.59%,可溶性固形物增长23.77%~37.43%,谷胱甘肽过氧化物酶(GSH-Px)及基因表达量分别增长了10.23%~110.41%和8.18%~123.91%,总硒含量提高3.09~34.14倍,硒代蛋氨酸(SeMet)增长100.00%~346.15%,半胱氨酸脱硫酶(CysD)基因表达量增长18.13%~195.43%。【结论】喷施纳米硒、亚硒酸钠和有机硒后转化为硒代氨基酸,且不同形态硒对灰枣的外观形态、抗氧化能力、硒代氨基酸均有影响。有机硒处理优于亚硒酸钠和纳米硒处理。

关键词: 灰枣, 抗氧化能力, 硒代谢

CLC Number:

S665.1

YUAN Yuting, LIU Defen, SUN Wanjin, JIA Wenzhao, MA Shengjun, KANG Lu. Study on the mechanism of different forms of selenium on antioxidant ability and selenium metabolism of Hui jujube[J]. Xinjiang Agricultural Sciences, 2025, 62(5): 1159-1169.

袁玉婷, 刘德粉, 孙万金, 贾文昭, 马生军, 康露. 不同形态硒调控灰枣抗氧化能力和硒代谢的机理分析[J]. 新疆农业科学, 2025, 62(5): 1159-1169.

Figures/Tables 9

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化合物 Compound	CAS	母离子 Parent ion (m/z)	子离子 Ions (m/z)	锥孔电压 Cone voltage (V)	碰撞能量 Collision energy (V)	保留时间 Retention time (min)	标准曲线 standard curve	R²
甲基硒代半胱氨酸 MeSeCys	26046-90-2	198.00	56.10*,88.10	20	15,10	0.98	y=341 830x-373.20	0.999 8
硒代胱氨酸 SeCys	29621-88-3	240	56.10*,98.10	20	20,15	1.01	y=225.67x-0.40	0.999 2
硒代蛋氨酸 SeMet	3211-76-5	184.2	166.90*,94.90	25	10,16	0.72	y=49605x-59.80	0.998 4

化合物 Compound	CAS	母离子 Parent ion (m/z)	子离子 Ions (m/z)	锥孔电压 Cone voltage (V)	碰撞能量 Collision energy (V)	保留时间 Retention time (min)	标准曲线 standard curve	R²
甲基硒代半胱氨酸 MeSeCys	26046-90-2	198.00	56.10*,88.10	20	15,10	0.98	y=341 830x-373.20	0.999 8
硒代胱氨酸 SeCys	29621-88-3	240	56.10*,98.10	20	20,15	1.01	y=225.67x-0.40	0.999 2
硒代蛋氨酸 SeMet	3211-76-5	184.2	166.90*,94.90	25	10,16	0.72	y=49605x-59.80	0.998 4

基因名称Gene name	Forward primer(5' to 3')	Reverse primer(5' to 3')
SOD	ACGGGTAATGTTTCTGGTCTCA	TGTTGTTTTCCAGCAGGGTT
POD	GTTCTGGGGTGTGATGC	GTATTCGGTTTGCGGTT
CAT	GCCATTCTATCGTCGTATCCA	ATCACAGTCACGCCACTCAGG
GSH-Px	CGTCTTCCATCATCGCTTCTTCA	AACAGCATACATTGTAGCTCCTC
APS	CCGATTGTGCTCGCCATTGA	GCCGGAACCTGTCAAGACCATC
APR	ATGGCTTTGRCTTTCACTTCTTC	CAACCTCCCAGTATCAAGGCTGA
SMT	GACGGCGGACTTGCGACGGA	GGCACCAGCTTCAAGGTAATC
CysD	TGGGCTTGGAGCTGCAATTGA	AGGCTAGCTCGTGCACTTGCA
ACT1	AGCCTTCCTGCCAACGAGT	TTGCTTCTCACCCTTGATGC

基因名称Gene name	Forward primer(5' to 3')	Reverse primer(5' to 3')
SOD	ACGGGTAATGTTTCTGGTCTCA	TGTTGTTTTCCAGCAGGGTT
POD	GTTCTGGGGTGTGATGC	GTATTCGGTTTGCGGTT
CAT	GCCATTCTATCGTCGTATCCA	ATCACAGTCACGCCACTCAGG
GSH-Px	CGTCTTCCATCATCGCTTCTTCA	AACAGCATACATTGTAGCTCCTC
APS	CCGATTGTGCTCGCCATTGA	GCCGGAACCTGTCAAGACCATC
APR	ATGGCTTTGRCTTTCACTTCTTC	CAACCTCCCAGTATCAAGGCTGA
SMT	GACGGCGGACTTGCGACGGA	GGCACCAGCTTCAAGGTAATC
CysD	TGGGCTTGGAGCTGCAATTGA	AGGCTAGCTCGTGCACTTGCA
ACT1	AGCCTTCCTGCCAACGAGT	TTGCTTCTCACCCTTGATGC

处理 Treatments	纵径 Vertical diameter (cm)	横径 Transverse diameter (cm)	硬度 Hardness (kg/cm²)
CK	3.61±0.18^a	2.34±0.20^ab	9.65±0.75^a
T₁	3.64±1.34^a	2.38±0.59^a	10.19±1.16^a
T₂	3.49±0.33^a	2.25±0.26^c	11.54±1.30^a
T₃	3.75±1.50^a	2.29±0.40^bc	11.45±1.50^a

Study on the mechanism of different forms of selenium on antioxidant ability and selenium metabolism of Hui jujube

不同形态硒调控灰枣抗氧化能力和硒代谢的机理分析

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Figures/Tables 9

References 36

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时间 Time (d)	处理 Treat- ments	灰枣叶片硒代氨基酸含量 Content of selenoamino acids in leaves of Hui jujube(μg/L)			灰枣果实硒代氨基酸含量 Content of selenoamino acids in Hui jujube fruit(μg/L)
时间 Time (d)	处理 Treat- ments	MeSeCys	SeCys	SeMet	MeSeCys	SeCys	SeMet
1	CK	0.002 4±0.000 1^a	0.006 2±0.000 1^b	0.018 3±0.003 0^c	0.001 4±0.000 1^ab	0.002 3±0.000 1^c	0.014 3±0.000 8^a
	T₁	0.002 1±0.000 1^b	0.007 7±0.002 6^b	0.030 3±0.000 3^b	0.001 2±0.000 2^b	0.016 5±0.005 1^ab	0.009 8±0.000 7^c
	T₂	0.001 6±0.000 1^c	0.010 6±0.000 1^a	0.029 1±0.001 8^b	0.001 4±0.000 2^b	0.010 6±0.004 4^b	0.013 1±0.001 3^a
	T₃	0.002 0±0.000 1^b	0.006 2±0.000 0^b	0.041 2±0.000 6^a	0.001 2±0.000 1^c	0.022 5±0.002 6^a	0.010 0±0.000 5^a
3	CK	0.002 0±0.000 1^c	0.012 1±0.002 6^a	0.027 3±0.001 9^b	0.001 4±0.000 0^a	0.007 7±0.002 6^c	0.013 5±0.000 6^b
	T₁	0.001 9±0.000 1^c	0.009 2±0.002 6^a	0.032 7±0.000 3^a	0.001 3±0.000 2^a	0.022 5±0.002 6^b	0.009 8±0.000 7^c
	T₂	0.002 4±0.000 1^a	0.013 6±0.002 6^a	0.032 4±0.000 6^a	0.001 3±0.000 1^b	0.026 9±0.002 6^b	0.014 6±0.001 8^b
	T₃	0.002 2±0.000 1^b	0.013 6±0.002 6^a	0.034 3±0.000 4^a	0.001 3±0.000 1^b	0.034 3±0.002 6^a	0.016 8±0.000 7^a
6	CK	0.001 7±0.000 2^b	0.012 4±0.001 6^a	0.028 9±0.000 7^c	0.001 4±0.000 1^ab	0.004 7±0.002 6^c	0.012 5±0.000 3^b
	T₁	0.001 8±0.000 2^b	0.013 6±0.002 6^a	0.032 1±0.001 1^b	0.001 2±0.000 1^b	0.035 7±0.002 6^a	0.013 4±0.000 3^b
	T₂	0.002 1±0.000 1^a	0.006 2±0.000 1^b	0.037 7±0.013 8^a	0.001 8±0.000 5^a	0.018 0±0.002 6^b	0.012 7±0.000 8^b
	T₃	0.001 7±0.000 2^b	0.013 6±0.002 6^a	0.024 0±0.000 2^d	0.001 2±0.000 1^b	0.020 0±0.000 9^b	0.014 9±0.000 9^ab
10	CK	-			0.001 3±0.000 1^a	0.003 2±0.002 6^b	0.011 4±0.000 7^b
	T₁				0.001 2±0.000 1^a	0.016 5±0.002 6^a	0.013 4±0.000 3^a
	T₂				0.001 7±0.000 9^a	0.012 1±0.002 6^a	0.012 6±0.000 7^b
	T₃				0.001 2±0.000 1^a	0.016 5±0.002 6^a	0.013 6±0.000 4^a

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