Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (5): 1292-1300.DOI: 10.6048/j.issn.1001-4330.2024.05.028
• Prataculture·Animal Husbandry Veterinarian • Previous Articles
FAN Yuexuan1,2(), MA Jiamei1,2, HUANG Chunyuan2, ZHENG Jiaxin2, CHEN Suzhen1,2, ZHANG Yan2, LIU Guangliang1,3(
), CAO Zongxi1,2(
)
Received:
2023-10-12
Online:
2024-05-20
Published:
2024-07-09
Correspondence author:
LIU Guangliang, CAO Zongxi
Supported by:
范悦轩1,2(), 马佳镁1,2, 黄春媛2, 郑佳馨2, 陈素贞1,2, 张艳2, 刘光亮1,3(
), 曹宗喜1,2(
)
通讯作者:
刘光亮,曹宗喜
作者简介:
范悦轩(1997-),男,新疆奇台人,硕士研究生,研究方向为家畜传染病学,(E-mial)1245219231@qq.com
基金资助:
CLC Number:
FAN Yuexuan, MA Jiamei, HUANG Chunyuan, ZHENG Jiaxin, CHEN Suzhen, ZHANG Yan, LIU Guangliang, CAO Zongxi. Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan[J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1292-1300.
范悦轩, 马佳镁, 黄春媛, 郑佳馨, 陈素贞, 张艳, 刘光亮, 曹宗喜. PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析[J]. 新疆农业科学, 2024, 61(5): 1292-1300.
项目 Items | 引物名称 Name of primer | 引物序列 Primer sequences | 扩增长度 Amplification length |
---|---|---|---|
NSP2 | NSP2-F | 5'-TCGGTGCACGTGTACCC-3' | 1 968 |
NSP3-R | 5'-GGCGGGGAAACTCTCTC-3' | ||
ORF5 | ORF5-F | 5'-ATGTTGGGGAAGTGCTTGACCGC-3' | 602 |
ORF5-R | 5'-CTAGAGACGACCCCATTGTTC-3' | ||
ORF7 | ORF7-F | 5'-GAATTCATGCCAAATAACAACGGC-3' | 372 |
ORF7-R | 5'-TCTAGATCATGTTGGGGGTGATGC-3' |
Tab.1 Primer of target fragment of PRRSV
项目 Items | 引物名称 Name of primer | 引物序列 Primer sequences | 扩增长度 Amplification length |
---|---|---|---|
NSP2 | NSP2-F | 5'-TCGGTGCACGTGTACCC-3' | 1 968 |
NSP3-R | 5'-GGCGGGGAAACTCTCTC-3' | ||
ORF5 | ORF5-F | 5'-ATGTTGGGGAAGTGCTTGACCGC-3' | 602 |
ORF5-R | 5'-CTAGAGACGACCCCATTGTTC-3' | ||
ORF7 | ORF7-F | 5'-GAATTCATGCCAAATAACAACGGC-3' | 372 |
ORF7-R | 5'-TCTAGATCATGTTGGGGGTGATGC-3' |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 99.2 | 99.2 | 99.1 | 98.7 | 85.2 | 99.2 | 99.6 | 99.0 | 95.5 | 95.8 | 92.6 | 93.5 | 92.6 | 86.0 | 86.0 | 86.3 | 86.4 | 85.9 | 86.5 | 87.0 | |
2 | 98.4 | 100.0 | 99.9 | 99.2 | 85.7 | 100.0 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 93.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
3 | 98.4 | 100.0 | 99.9 | 99.2 | 85.7 | 100.0 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 93.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
4 | 98.4 | 100.0 | 100.0 | 99.1 | 85.6 | 99.9 | 99.5 | 99.6 | 96.1 | 96.5 | 93.3 | 94.2 | 93.0 | 86.4 | 89.2 | 86.6 | 86.8 | 86.3 | 86.6 | 87.4 | |
5 | 98.4 | 100.0 | 100.0 | 100.0 | 85.0 | 99.2 | 99.1 | 99.0 | 95.8 | 96.2 | 92.9 | 93.8 | 92.6 | 85.7 | 89.4 | 86.3 | 86.4 | 85.7 | 86.0 | 86.8 | |
6 | 84.0 | 85.2 | 85.2 | 85.2 | 85.2 | 85.7 | 85.6 | 85.7 | 85.7 | 86.0 | 86.8 | 85.1 | 86.3 | 95.6 | 85.0 | 95.2 | 94.6 | 94.7 | 94.2 | 94.2 | |
7 | 98.4 | 100.0 | 100.0 | 100.0 | 100.0 | 85.2 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 96.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
8 | 98.8 | 99.6 | 99.6 | 99.6 | 99.6 | 84.8 | 99.6 | 99.4 | 95.8 | 96.2 | 93.0 | 93.9 | 93.0 | 86.4 | 89.5 | 86.6 | 86.8 | 86.3 | 86.6 | 87.4 | |
9 | 97.7 | 99.2 | 99.2 | 99.2 | 99.2 | 85.2 | 99.2 | 98.8 | 96.0 | 96.4 | 93.1 | 94.0 | 92.9 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 87.0 | 87.5 | |
10 | 94.6 | 95.7 | 95.7 | 95.7 | 95.7 | 85.6 | 95.7 | 95.3 | 94.9 | 99.1 | 94.8 | 95.6 | 95.1 | 87.3 | 90.0 | 86.9 | 87.7 | 86.5 | 87.5 | 88.1 | |
11 | 94.9 | 96.5 | 96.5 | 96.5 | 96.5 | 86.0 | 96.5 | 96.1 | 95.7 | 98.4 | 95.1 | 96.2 | 94.8 | 87.5 | 90.3 | 87.2 | 87.9 | 86.7 | 87.3 | 88.1 | |
12 | 91.8 | 93.4 | 93.4 | 93.4 | 93.4 | 86.4 | 93.4 | 93.0 | 92.6 | 93.0 | 94.2 | 92.6 | 95.5 | 87.7 | 90.1 | 87.3 | 87.0 | 87.4 | 87.7 | 87.4 | |
13 | 92.2 | 93.8 | 93.8 | 93.8 | 93.8 | 82.9 | 93.8 | 93.4 | 93.0 | 94.6 | 95.7 | 90.7 | 92.6 | 86.5 | 88.6 | 86.5 | 87.2 | 85.5 | 86.0 | 86.9 | |
14 | 92.6 | 93.0 | 93.0 | 93.0 | 93.0 | 84.0 | 93.0 | 93.4 | 92.2 | 94.9 | 94.6 | 93.0 | 92.2 | 87.3 | 89.9 | 86.9 | 86.9 | 87.1 | 87.5 | 87.0 | |
15 | 85.2 | 86.4 | 86.4 | 86.4 | 86.4 | 94.2 | 86.4 | 86.0 | 86.4 | 88.3 | 88.7 | 87.9 | 86.4 | 86.4 | 85.7 | 96.5 | 95.6 | 95.7 | 95.6 | 96.0 | |
16 | 86.8 | 87.5 | 87.5 | 87.5 | 87.5 | 83.7 | 87.5 | 87.9 | 87.5 | 88.3 | 88.7 | 89.5 | 86.8 | 89.1 | 86.0 | 85.3 | 85.5 | 85.8 | 85.6 | 86.3 | |
17 | 86.4 | 87.5 | 87.5 | 87.5 | 87.5 | 94.2 | 87.5 | 87.2 | 87.5 | 88.3 | 88.7 | 88.7 | 85.6 | 87.5 | 96.1 | 85.6 | 94.4 | 95.2 | 95.2 | 95.7 | |
18 | 86.4 | 87.5 | 87.5 | 87.5 | 87.5 | 93.0 | 87.5 | 87.2 | 87.5 | 89.1 | 89.5 | 87.9 | 86.4 | 87.2 | 95.3 | 86.8 | 93.4 | 95.1 | 94.6 | 94.6 | |
19 | 85.9 | 86.3 | 86.3 | 86.3 | 86.3 | 93.4 | 86.3 | 86.7 | 86.3 | 88.3 | 88.7 | 88.7 | 85.5 | 88.3 | 96.1 | 87.9 | 95.7 | 94.1 | 95.1 | 94.4 | |
20 | 87.9 | 88.3 | 88.3 | 88.3 | 88.3 | 92.6 | 88.3 | 87.9 | 89.1 | 89.1 | 88.7 | 88.3 | 86.0 | 87.5 | 94.6 | 86.4 | 95.3 | 94.2 | 94.9 | 94.8 | |
21 | 85.6 | 86.8 | 86.8 | 86.8 | 86.8 | 91.8 | 86.8 | 86.4 | 86.8 | 88.3 | 87.9 | 87.2 | 84.8 | 86.4 | 95.3 | 87.2 | 94.6 | 93.4 | 94.5 | 94.6 |
Tab.2 Comparison of nucleotide and amino acid sequences of ORF5 gene between PRRSV HNHK3-2021 strain and other strain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 99.2 | 99.2 | 99.1 | 98.7 | 85.2 | 99.2 | 99.6 | 99.0 | 95.5 | 95.8 | 92.6 | 93.5 | 92.6 | 86.0 | 86.0 | 86.3 | 86.4 | 85.9 | 86.5 | 87.0 | |
2 | 98.4 | 100.0 | 99.9 | 99.2 | 85.7 | 100.0 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 93.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
3 | 98.4 | 100.0 | 99.9 | 99.2 | 85.7 | 100.0 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 93.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
4 | 98.4 | 100.0 | 100.0 | 99.1 | 85.6 | 99.9 | 99.5 | 99.6 | 96.1 | 96.5 | 93.3 | 94.2 | 93.0 | 86.4 | 89.2 | 86.6 | 86.8 | 86.3 | 86.6 | 87.4 | |
5 | 98.4 | 100.0 | 100.0 | 100.0 | 85.0 | 99.2 | 99.1 | 99.0 | 95.8 | 96.2 | 92.9 | 93.8 | 92.6 | 85.7 | 89.4 | 86.3 | 86.4 | 85.7 | 86.0 | 86.8 | |
6 | 84.0 | 85.2 | 85.2 | 85.2 | 85.2 | 85.7 | 85.6 | 85.7 | 85.7 | 86.0 | 86.8 | 85.1 | 86.3 | 95.6 | 85.0 | 95.2 | 94.6 | 94.7 | 94.2 | 94.2 | |
7 | 98.4 | 100.0 | 100.0 | 100.0 | 100.0 | 85.2 | 99.6 | 99.7 | 96.2 | 96.6 | 93.4 | 94.3 | 96.1 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 86.8 | 87.5 | |
8 | 98.8 | 99.6 | 99.6 | 99.6 | 99.6 | 84.8 | 99.6 | 99.4 | 95.8 | 96.2 | 93.0 | 93.9 | 93.0 | 86.4 | 89.5 | 86.6 | 86.8 | 86.3 | 86.6 | 87.4 | |
9 | 97.7 | 99.2 | 99.2 | 99.2 | 99.2 | 85.2 | 99.2 | 98.8 | 96.0 | 96.4 | 93.1 | 94.0 | 92.9 | 86.5 | 89.4 | 86.8 | 86.9 | 86.2 | 87.0 | 87.5 | |
10 | 94.6 | 95.7 | 95.7 | 95.7 | 95.7 | 85.6 | 95.7 | 95.3 | 94.9 | 99.1 | 94.8 | 95.6 | 95.1 | 87.3 | 90.0 | 86.9 | 87.7 | 86.5 | 87.5 | 88.1 | |
11 | 94.9 | 96.5 | 96.5 | 96.5 | 96.5 | 86.0 | 96.5 | 96.1 | 95.7 | 98.4 | 95.1 | 96.2 | 94.8 | 87.5 | 90.3 | 87.2 | 87.9 | 86.7 | 87.3 | 88.1 | |
12 | 91.8 | 93.4 | 93.4 | 93.4 | 93.4 | 86.4 | 93.4 | 93.0 | 92.6 | 93.0 | 94.2 | 92.6 | 95.5 | 87.7 | 90.1 | 87.3 | 87.0 | 87.4 | 87.7 | 87.4 | |
13 | 92.2 | 93.8 | 93.8 | 93.8 | 93.8 | 82.9 | 93.8 | 93.4 | 93.0 | 94.6 | 95.7 | 90.7 | 92.6 | 86.5 | 88.6 | 86.5 | 87.2 | 85.5 | 86.0 | 86.9 | |
14 | 92.6 | 93.0 | 93.0 | 93.0 | 93.0 | 84.0 | 93.0 | 93.4 | 92.2 | 94.9 | 94.6 | 93.0 | 92.2 | 87.3 | 89.9 | 86.9 | 86.9 | 87.1 | 87.5 | 87.0 | |
15 | 85.2 | 86.4 | 86.4 | 86.4 | 86.4 | 94.2 | 86.4 | 86.0 | 86.4 | 88.3 | 88.7 | 87.9 | 86.4 | 86.4 | 85.7 | 96.5 | 95.6 | 95.7 | 95.6 | 96.0 | |
16 | 86.8 | 87.5 | 87.5 | 87.5 | 87.5 | 83.7 | 87.5 | 87.9 | 87.5 | 88.3 | 88.7 | 89.5 | 86.8 | 89.1 | 86.0 | 85.3 | 85.5 | 85.8 | 85.6 | 86.3 | |
17 | 86.4 | 87.5 | 87.5 | 87.5 | 87.5 | 94.2 | 87.5 | 87.2 | 87.5 | 88.3 | 88.7 | 88.7 | 85.6 | 87.5 | 96.1 | 85.6 | 94.4 | 95.2 | 95.2 | 95.7 | |
18 | 86.4 | 87.5 | 87.5 | 87.5 | 87.5 | 93.0 | 87.5 | 87.2 | 87.5 | 89.1 | 89.5 | 87.9 | 86.4 | 87.2 | 95.3 | 86.8 | 93.4 | 95.1 | 94.6 | 94.6 | |
19 | 85.9 | 86.3 | 86.3 | 86.3 | 86.3 | 93.4 | 86.3 | 86.7 | 86.3 | 88.3 | 88.7 | 88.7 | 85.5 | 88.3 | 96.1 | 87.9 | 95.7 | 94.1 | 95.1 | 94.4 | |
20 | 87.9 | 88.3 | 88.3 | 88.3 | 88.3 | 92.6 | 88.3 | 87.9 | 89.1 | 89.1 | 88.7 | 88.3 | 86.0 | 87.5 | 94.6 | 86.4 | 95.3 | 94.2 | 94.9 | 94.8 | |
21 | 85.6 | 86.8 | 86.8 | 86.8 | 86.8 | 91.8 | 86.8 | 86.4 | 86.8 | 88.3 | 87.9 | 87.2 | 84.8 | 86.4 | 95.3 | 87.2 | 94.6 | 93.4 | 94.5 | 94.6 |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 100.0 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.4 | 99.5 | 90.6 | 91.9 | |
2 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 87.9 | 99.5 | 90.6 | 91.9 | |
3 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 87.9 | 99.5 | 90.6 | 91.9 | |
4 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.8 | 91.4 | 89.0 | 88.4 | 99.5 | 91.1 | 92.5 | |
5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.2 | 100.0 | 90.6 | 92.2 | |
6 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.8 | 90.9 | 88.4 | 88.4 | 99.5 | 91.1 | 91.9 | |
7 | 100.0 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.4 | 99.5 | 90.6 | 91.9 | |
8 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 99.2 | 93.0 | 95.7 | 95.7 | 93.5 | 93.0 | 89.2 | 90.6 | 88.4 | 87.9 | 98.9 | 90.6 | 91.4 | |
9 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.2 | 93.5 | 96.2. | 96.2 | 93.8 | 93.5 | 89.5 | 91.1 | 88.7 | 88.2 | 99.7 | 90.9 | 92.2 | |
10 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.0 | 93.5 | 94.1 | 94.4 | 93.3 | 90.9 | 91.7 | 92.7 | 89.8 | 90.3 | 93.3 | 91.4 | 92.2 | |
11 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96 | 95.7 | 96.2 | 94.1 | 99.5 | 95.4 | 96.0 | 90.6 | 92.2 | 89.2 | 89.8 | 96.0 | 91.4 | 91.1 | |
12 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96 | 95.7 | 96.2 | 94.4 | 99.5 | 95.4 | 96.5 | 90.9 | 92.2 | 89.2 | 90.1 | 96.0 | 91.4 | 91.1 | |
13 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.8 | 93.3 | 95.4 | 95.4 | 92.7 | 89.8 | 90.9 | 89.0 | 88.7 | 93.5 | 90.1 | 89.5 | |
14 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.0 | 93.5 | 90.9 | 96.0 | 96.5 | 92.7 | 89.0 | 90.3 | 87.9 | 88.2 | 93.3 | 90.1 | 90.3 | |
15 | 89.2 | 89.2 | 89.2 | 89.8 | 89.2 | 89.8 | 89.2 | 89.2 | 89.5 | 91.7 | 90.6 | 90.9 | 89.8 | 89.0 | 97.8 | 94.4 | 90.0 | 89.2 | 97.3 | 90.9 | |
16 | 90.9 | 90.9 | 90.9 | 91.4 | 90.9 | 90.9 | 90.9 | 90.6 | 91.1 | 92.7 | 92.2 | 92.2 | 90.9 | 90.5 | 97.4 | 95.4 | 96.5 | 90.9 | 97.8 | 92.5 | |
17 | 88.4 | 88.4 | 88.4 | 89.0 | 88.4 | 88.4 | 88.4 | 88.4 | 88.7 | 89.8 | 89.2 | 89.2 | 89.0 | 87.9 | 94.4 | 95.4 | 94.6 | 88.4 | 94.9 | 88.4 | |
18 | 88.4 | 87.9 | 87.9 | 88.4 | 88.2 | 88.4 | 88.4 | 87.9 | 88.2 | 90.3 | 89.8 | 90.1 | 88.7 | 88.2 | 97.0 | 96.5 | 94.6 | 88.2 | 96.5 | 90.1 | |
19 | 99.5 | 99.5 | 99.5 | 99.5 | 100.0 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 86.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.2 | 90.6 | 92.2 | |
20 | 90.6 | 90.6 | 90.6 | 91.1 | 90.6 | 91.1 | 90.6 | 90.6 | 90.9 | 91.4 | 91.4 | 91.4 | 90.1 | 90.1 | 97.3 | 97.8 | 94.9 | 96.5 | 90.6 | 91.9 | |
21 | 91.9 | 91.9 | 91.9 | 92.5 | 92.2 | 91.9 | 91.9 | 91.4 | 92.2 | 92.2 | 91.1 | 91.1 | 89.5 | 90.3 | 80.9 | 92.5 | 88.4 | 90.1 | 92.2 | 91.9 |
Tab.3 Comparison of nucleotide and amino acid sequences of ORF7 gene between PRRSV HNHK3-2021 strain and other strains
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 100.0 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.4 | 99.5 | 90.6 | 91.9 | |
2 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 87.9 | 99.5 | 90.6 | 91.9 | |
3 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 87.9 | 99.5 | 90.6 | 91.9 | |
4 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.8 | 91.4 | 89.0 | 88.4 | 99.5 | 91.1 | 92.5 | |
5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.2 | 100.0 | 90.6 | 92.2 | |
6 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.8 | 90.9 | 88.4 | 88.4 | 99.5 | 91.1 | 91.9 | |
7 | 100.0 | 99.5 | 99.5 | 99.5 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 96.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.4 | 99.5 | 90.6 | 91.9 | |
8 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 98.9 | 99.2 | 93.0 | 95.7 | 95.7 | 93.5 | 93.0 | 89.2 | 90.6 | 88.4 | 87.9 | 98.9 | 90.6 | 91.4 | |
9 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.7 | 99.2 | 93.5 | 96.2. | 96.2 | 93.8 | 93.5 | 89.5 | 91.1 | 88.7 | 88.2 | 99.7 | 90.9 | 92.2 | |
10 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.0 | 93.5 | 94.1 | 94.4 | 93.3 | 90.9 | 91.7 | 92.7 | 89.8 | 90.3 | 93.3 | 91.4 | 92.2 | |
11 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96 | 95.7 | 96.2 | 94.1 | 99.5 | 95.4 | 96.0 | 90.6 | 92.2 | 89.2 | 89.8 | 96.0 | 91.4 | 91.1 | |
12 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96.0 | 96 | 95.7 | 96.2 | 94.4 | 99.5 | 95.4 | 96.5 | 90.9 | 92.2 | 89.2 | 90.1 | 96.0 | 91.4 | 91.1 | |
13 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.5 | 93.8 | 93.3 | 95.4 | 95.4 | 92.7 | 89.8 | 90.9 | 89.0 | 88.7 | 93.5 | 90.1 | 89.5 | |
14 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.3 | 93.0 | 93.5 | 90.9 | 96.0 | 96.5 | 92.7 | 89.0 | 90.3 | 87.9 | 88.2 | 93.3 | 90.1 | 90.3 | |
15 | 89.2 | 89.2 | 89.2 | 89.8 | 89.2 | 89.8 | 89.2 | 89.2 | 89.5 | 91.7 | 90.6 | 90.9 | 89.8 | 89.0 | 97.8 | 94.4 | 90.0 | 89.2 | 97.3 | 90.9 | |
16 | 90.9 | 90.9 | 90.9 | 91.4 | 90.9 | 90.9 | 90.9 | 90.6 | 91.1 | 92.7 | 92.2 | 92.2 | 90.9 | 90.5 | 97.4 | 95.4 | 96.5 | 90.9 | 97.8 | 92.5 | |
17 | 88.4 | 88.4 | 88.4 | 89.0 | 88.4 | 88.4 | 88.4 | 88.4 | 88.7 | 89.8 | 89.2 | 89.2 | 89.0 | 87.9 | 94.4 | 95.4 | 94.6 | 88.4 | 94.9 | 88.4 | |
18 | 88.4 | 87.9 | 87.9 | 88.4 | 88.2 | 88.4 | 88.4 | 87.9 | 88.2 | 90.3 | 89.8 | 90.1 | 88.7 | 88.2 | 97.0 | 96.5 | 94.6 | 88.2 | 96.5 | 90.1 | |
19 | 99.5 | 99.5 | 99.5 | 99.5 | 100.0 | 99.5 | 99.5 | 98.9 | 99.7 | 93.3 | 96.0 | 86.0 | 93.5 | 93.3 | 89.2 | 90.9 | 88.4 | 88.2 | 90.6 | 92.2 | |
20 | 90.6 | 90.6 | 90.6 | 91.1 | 90.6 | 91.1 | 90.6 | 90.6 | 90.9 | 91.4 | 91.4 | 91.4 | 90.1 | 90.1 | 97.3 | 97.8 | 94.9 | 96.5 | 90.6 | 91.9 | |
21 | 91.9 | 91.9 | 91.9 | 92.5 | 92.2 | 91.9 | 91.9 | 91.4 | 92.2 | 92.2 | 91.1 | 91.1 | 89.5 | 90.3 | 80.9 | 92.5 | 88.4 | 90.1 | 92.2 | 91.9 |
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