• Article • Previous Articles Next Articles
FENG Chun-yan;QING De-gang;NI Hui;SUN Yu;ZHANG Juan;JIA Xiao-guang;XU Jian-guo
Received:
Revised:
Online:
Published:
冯春艳;卿德刚;倪慧;孙宇;张娟;贾晓光;徐建国
Abstract: [Objective] To determine the content of rutin, hyperoside and isoquercitrin in apocynum leaf by HPLC, and compare the content of 3 flavonoids in Apocynum leaf from different sources.[Method]The assay was performed on symmetry column (250 mm×4.6 mm, 5 μm), eluted with a gradient of acetonitrile-0.2; aqueous phosphoric acid at 35℃.The rate of flow was 1.0 mL/min, detection wavelength was 360 nm, the infection volume was 10 μL.[Result]The linear ranges of rutin, hyperoside and isoquercitrin were 0.251,1-1.255,4 μg, 0.026,6-0.265,9 μg, and 0.235,6-1.178,0 μg, and the correlation coefficients were 0.999,8, 0.999,9 and 0.999,9 respectively;The average recovery rates were 103.6;, 102.1; and 104.4;, respectively and the RSD was less than 3.8;.The content of isoquercitrin in Poacynum hendersonii(Hook.f.) Woodson was the highest,rutin in Apocynum venetum Linn was the highest ,and hypericides were detected only in Apocynum venetum Linn.[Conclusion]HPLC method can be used to determine the content of 3 flavonoids in Apocynum venetum L.There are certain rules in the content of 3 flavonoids in two kinds of Apocynum venetum, and the results can provide two methods and basis for quality control of Apocynum venetum, The content of isoquercitrin in Apocynum venetum Linn is slightly higher than that of Poacynum hendersonii (Hook.f.) Woodson, the content of hyperoside is only 0.05;, no hypericides were detected in Poacynum hendersonii (Hook.f.) Woodson, and the results can provide two methods and basis for quality control of Apocynm venetum.
摘要: [目的]建立并测定罗布麻叶中芦丁、金丝桃苷、异槲皮苷含量的HPLC方法,并比较2种罗布麻叶中3种黄酮成分的含量差异.为2种罗布麻质量控制提供方法和依据.[方法]采用Symmetry Shield(250 mm× 4.6 mm,5 μm)色谱柱,在35℃条件下以乙腈-0.2;磷酸水溶液梯度洗脱,流速为1.0 mL/min,检测波长为360 nm,进样量为10 μL.[结果]芦丁、金丝桃苷、异槲皮苷的线性范围分别为0.251 1~1.255 4 μg、0.026 6~0.265 9 μg、0.235 6~1.178 0 μg,相关系数R分别为0.999 8、0.999 9、0.999 9;平均加标回收率分别为103.6;、102.1;、104.4;,RSD均小于3.8;.大叶白麻中异槲皮苷含量最高,罗布红麻中芦丁含量最高,并且仅有罗布红麻中检测到了金丝桃苷.[结论]HPLC测定方法可用于罗布麻叶中3种黄酮成分的含量测定,2种罗布麻叶中3种黄酮成分含量差异有一定规律,罗布红麻中异槲皮苷含量略高于大叶白麻,芦丁含量则至少是大叶白麻的13倍,金丝桃苷含量仅为0.05;,且在大叶白麻中未检测到金丝桃苷,研究结果可为2种罗布麻质量控制提供方法和依据.
FENG Chun-yan;QING De-gang;NI Hui;SUN Yu;ZHANG Juan;JIA Xiao-guang;XU Jian-guo. Comparative Study on the Contents of Rutin, Hyperoside and Isoquercitrin in Two Kinds of Apocynum Leaves[J]. .
冯春艳;卿德刚;倪慧;孙宇;张娟;贾晓光;徐建国. 2种罗布麻叶中芦丁、金丝桃苷、异槲皮苷含量比较研究[J]. .
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.xjnykx.com/EN/
https://www.xjnykx.com/EN/Y2017/V54/I7/1291