]*>","")" /> Cloning and Expression of SerA in C-11 Producing L-serine

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Cloning and Expression of SerA in C-11 Producing L-serine

XIE Yu-qing;LUO Shu-ping;Mao Jun   

  • Received:2007-05-25 Revised:2007-05-25 Online:2007-05-25 Published:2007-05-25

L-丝氨酸产生菌c-11中serA基因的克隆与表达

谢玉清;罗淑萍;茆军   

  1. 新疆农业大学农学院,乌鲁木齐,830052;新疆农科院微生物所,乌鲁木齐,830091

摘要: 利用PCR技术以L-丝氨酸产生菌株黄色短杆菌c-11的染色体DNA为摸板,扩增出serA基因,连接到表达载体pEC7上,转化L-丝氨酸产生菌c-11,使其氨基酸产量由12 g/L增加到18 g/L,产酸率增加了50;.