]*>","")" /> Expression of endoglucanase CelG gene of Clostridium thermocellum in Bacillus subtilis

• Article • Previous Articles     Next Articles

Expression of endoglucanase CelG gene of Clostridium thermocellum in Bacillus subtilis

CHU Min;YANG Hong-mei;WANG Yun;LOU kai   

  • Received:2008-02-25 Revised:2008-02-25 Online:2008-02-25 Published:2008-02-25

热纤维素梭菌内切葡聚糖苷酶基因在枯草芽孢杆菌的表达

楚敏;杨红梅;王芸;娄恺   

  1. 新疆特殊环境微生物重点实验室/新疆农科院微生物所,乌鲁木齐,830091

摘要: 对热纤维素梭菌Clostridium thermocellum内切葡聚糖苷酶基因CelG及其信号肽序列进行了克隆及表达研究.以热纤维素梭菌总DNA为模板,经PCR扩增获得目的,并连接到大肠杆菌-枯草杆菌穿梭质粒pSW1载体,获得质粒pSW1-CelG;用感受态转化法将获得质粒pSW1-CelG转化到枯草芽孢杆菌BGSC中进行分泌表达,获得了大小约56KD的蛋白片段,且具有生物活性,证明CelG基因克隆成功并正确表达.