• Article • Previous Articles Next Articles
CHU Min;YANG Hong-mei;WANG Yun;LOU kai
Received:
Revised:
Online:
Published:
楚敏;杨红梅;王芸;娄恺
摘要: 对热纤维素梭菌Clostridium thermocellum内切葡聚糖苷酶基因CelG及其信号肽序列进行了克隆及表达研究.以热纤维素梭菌总DNA为模板,经PCR扩增获得目的,并连接到大肠杆菌-枯草杆菌穿梭质粒pSW1载体,获得质粒pSW1-CelG;用感受态转化法将获得质粒pSW1-CelG转化到枯草芽孢杆菌BGSC中进行分泌表达,获得了大小约56KD的蛋白片段,且具有生物活性,证明CelG基因克隆成功并正确表达.
CHU Min;YANG Hong-mei;WANG Yun;LOU kai. Expression of endoglucanase CelG gene of Clostridium thermocellum in Bacillus subtilis[J]. .
楚敏;杨红梅;王芸;娄恺. 热纤维素梭菌内切葡聚糖苷酶基因在枯草芽孢杆菌的表达[J]. .
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.xjnykx.com/EN/
https://www.xjnykx.com/EN/Y2008/V45/I2/333