]*>","")" /> Cloning the HSP20 Gene of B.bigemina Isolated in Xinjiang and Construction of the Eukaryotic Expression Plasmid
Xinjiang Agricultural Sciences

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Cloning the HSP20 Gene of B.bigemina Isolated in Xinjiang and Construction of the Eukaryotic Expression Plasmid

JIAN Zi-jian;SUN Qi-zhe;MA Su-zhen;WANG Xiao-ping   

  • Received:2009-02-25 Revised:2009-02-25 Online:2009-02-25 Published:2009-02-25

牛双芽巴贝斯虫新疆株HSP20基因的克隆和真核表达质粒的构建

简子健;孙其喆;马素贞;王晓萍   

  1. 新疆农业大学动物医学学院,乌鲁木齐,830052;新疆农业大学继续教育学院,乌鲁木齐,830052





摘要： 通过设计牛双芽巴贝斯虫热休克蛋白HSP20基因序列的特异性引物,采用 PCR方法从疑似"牛焦虫病"的患牛全血基因组中扩增得到699 bp的HSP20基因,将其连入pMD18-T测序、鉴定,再将验证的699 bp的HSP20基因克隆至真核表达载体pcDNA3.1(+)中,构建真核载体PCDNA3.1-HSP20.经再次测序、鉴定后,尾静脉注射小白鼠进行HSP20基因的瞬时表达,取其肝脏提取总 RNA,经RT-PCR方法扩增出一条534 bp的目的条带,表明真核表达质粒构建成功.