]*>","")" /> The Preparation of Liposome Freeze-dried Oral Live Vaccines of Rabies Attenuated Strain and Its Physical and Chemical Property
Xinjiang Agricultural Sciences

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The Preparation of Liposome Freeze-dried Oral Live Vaccines of Rabies Attenuated Strain and Its Physical and Chemical Property

  • Received:2013-11-25 Revised:2013-11-25 Online:2013-11-25 Published:2013-11-25

狂犬病减毒脂质体口服冻干活疫苗的制备及理化性质的检测

翟少华;骆建敏;赵森;马素贞;高攀;苏晓慧;夏婷婷;张美玲;李建飞   

  1. 新疆农业大学动物医学学院,乌鲁木齐,830052;新疆大学理化测试中心,乌鲁木齐,830046





摘要： [目的]分析研发的狂犬病减毒脂质体口服冻干活疫苗的脂质体形态、粒径分布状态、疫苗抗原包封率,确定狂犬病减毒脂质体口服冻干活疫苗的制备工艺.[方法](1)以大豆卵磷脂、胆固醇、十八胺、VE等物质为原料,采用薄膜分散法制备空白脂质体,采用电子显微镜和激光粒径分布仪测定空白脂质体的形态与粒径分布;(2)采用冷冻干燥方法将狂犬病rSRV9减毒株与空白脂质体等体积混合后冻干,制备狂犬病减毒脂质体口服冻干活疫苗;(3)采用BCA蛋白定量法检测狂犬病减毒脂质体口服冻干活疫苗的抗原包封率.[结果]制备空白脂质体形态为大单室脂质体;平均粒径为378 nm;疫苗包封率为71.19;.[结论]薄膜分散-冻干方法制备狂犬病减毒脂质体口服冻干活疫苗,抗原包封率高、性状稳定.