]*>","")" /> Cloning and Prokaryotic Expression of Coat Protein Gene of Cucumber Mosaic Virus subgroup Ⅰ B Separated from Chilli Pepper in Xinjiang
Xinjiang Agricultural Sciences

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Cloning and Prokaryotic Expression of Coat Protein Gene of Cucumber Mosaic Virus subgroup Ⅰ B Separated from Chilli Pepper in Xinjiang

ZHANG Qiang;CHEN Ying-jie;XIANG Ben-chun;ZHENG Yin-ying   

  • Received:2014-12-25 Revised:2014-12-25 Online:2014-12-25 Published:2014-12-25

辣椒上CMV亚组ⅠB CP基因克隆与原核表达

张强;陈英杰;向本春;郑银英   

  1. 石河子大学生命科学学院,新疆石河子,832003





摘要： [目的]克隆辣椒上黄瓜花叶病毒外壳蛋白(CMV CP)基因,诱导CP蛋白表达,为下一步制备高效、特异的抗血清奠定基础.[方法]根据已知的新疆石河子辣椒上的CP基因序列设计引物,克隆CMV CP基因,经测序、Noc Ⅰ/BamH Ⅰ酶切鉴定,成功构建了原核表达载体pET-CMV CP,转化大肠杆菌BL21 DE3,用终浓度为1 mmol/L IPTG诱导蛋白表达,SDS-PAGE检测目的蛋白表达.[结果]成功构建了新疆辣椒上CMV CP原核表达载体pET-CMV CP,获得了与预期大小一致的30 kDa蛋白.[结论]新疆石河子辣椒上CMV CP在原核中获得了正确表达.