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ZHANG Qiang;CHEN Ying-jie;XIANG Ben-chun;ZHENG Yin-ying
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张强;陈英杰;向本春;郑银英
摘要: [目的]克隆辣椒上黄瓜花叶病毒外壳蛋白(CMV CP)基因,诱导CP蛋白表达,为下一步制备高效、特异的抗血清奠定基础.[方法]根据已知的新疆石河子辣椒上的CP基因序列设计引物,克隆CMV CP基因,经测序、Noc Ⅰ/BamH Ⅰ酶切鉴定,成功构建了原核表达载体pET-CMV CP,转化大肠杆菌BL21 DE3,用终浓度为1 mmol/L IPTG诱导蛋白表达,SDS-PAGE检测目的蛋白表达.[结果]成功构建了新疆辣椒上CMV CP原核表达载体pET-CMV CP,获得了与预期大小一致的30 kDa蛋白.[结论]新疆石河子辣椒上CMV CP在原核中获得了正确表达.
ZHANG Qiang;CHEN Ying-jie;XIANG Ben-chun;ZHENG Yin-ying. Cloning and Prokaryotic Expression of Coat Protein Gene of Cucumber Mosaic Virus subgroup Ⅰ B Separated from Chilli Pepper in Xinjiang[J]. .
张强;陈英杰;向本春;郑银英. 辣椒上CMV亚组ⅠB CP基因克隆与原核表达[J]. .
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https://www.xjnykx.com/EN/Y2014/V51/I12/2257