]*>","")" /> The Comparison of Immunohistochemistry and Serum ELISA to Detect Hepatic HEV Antigen in Cattle and Sheep
Xinjiang Agricultural Sciences

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The Comparison of Immunohistochemistry and Serum ELISA to Detect Hepatic HEV Antigen in Cattle and Sheep

Mamat Aizizi;ZHAI Shao-hua;SONG Zhen-hui;Ahmat o Mamat   

  • Received:2014-03-25 Revised:2014-03-25 Online:2014-03-25 Published:2014-03-25

牛羊肝脏中HEV抗原免疫组化检测法与血清ELISA检测法的比较

买买提·艾孜子;翟少华;宋振辉;阿合买提·买买提   

  1. 新疆农业大学动物医学院,乌鲁木齐,830052





摘要： [目的]比较牛羊肝脏中HEV抗原免疫组化法与血清ELISA法,验证免疫组化抗体符合率.[方法]用免疫组化法与血清ELISA法共检测牛血清和肝脏样品各87份,羊血清和肝脏样品各84份;比较牛羊肝脏中HEV抗原免疫组化法与血清ELISA法检测结果,x2检验.[结果]检测牛血清抗HEV-IgG抗体检测结果显示,阳性率达21.84;.制作免疫组化染色切片检测结果显示,阳性率达19.54;.羊血清抗HEV-IgG抗体检测结果显示,阳性率达14.29;.制作免疫组化染色切片检测结果显示,阳性率达11.90;.两种检测方法比较统计学分析表明,牛组x2=0.50,P＞0.05,羊组x2=0.50,P＞0.05,牛羊各组结果均不显著.[结论]免疫组化染色方法可以替代复杂的血清ELISA法,用于屠宰牛羊肝脏中HEV抗原的检测.