Abstract: [Objective] This paper analyzes the relation between relative body length,relative time and negative weight in the 1,600 m speed race,and preliminarily studies the handicap rule of Yili horse 1,600 m speed race.[Method]This study took 8 3-4 year old Yili horse as the research object,and carried on the 1,600 m speed race test,and horses in each race were 0 kg,0.5 kg,1 kg,1.5 kg,2 kg,2.5 kg,3 kg,3.5kg,4 kg,4.5 kg,5 kg,11 weight allowance,and this trial was repeated for each scale to analyze the relation between the relative body length,the relative time used,the score and the handicap in the race.[Result] Through the univariate analysis of the factorrs affecting the performance of the general linear model,it was found that the handicap had a significant influence on the result of competition (P ＜0.01),and other factorrs had no significant difference in the racing(P ＞ 0.05).By comparing the differences between the scores of the horses,and the difference of the lengths and the different time,it was found that there were most significant or significant differences (P ＜0.01 or P ＜0.05).By the correlation analysis among the handicaps,the lengths and the difference time in the racing,it was found that the handicap,lengths and the difference time was positively correlated with the difference (P ＜0.01).Through the regression model among the handicap,lengths and the difference time,it was found that the linear equations had great significance (P ＜ 0.01).[Conclusion] By establishing regression model,we find that the linear regression equation between negative pound and time difference was y =1.973 x,the linear regression equation between lengths and the difference time was y =0.230 x.Based on the regression model,the handicap can be calculated by the difference time between the lengths and the difference of the preceding tournament.By simulating comparing the variation coefficient of condition races and handicap race,the coefficient of variation of the handicap race is significantly lower than that of the condition races tournament.By comparing the use of the head (tail) horse and the intermediate horse as the reference to the horse effect,the use of intermediate horses as a reference horse can effectively enlarge the maximum score difference.