单稀释法间接ELISA和终点法中和试验检测犊牛轮状病毒疫苗抗体效价比较

单稀释法间接ELISA和终点法中和试验检测犊牛轮状病毒疫苗抗体效价比较

简子健;曹竹亭;马素贞;高文斌;张晓红;王晓萍;袁江玲

新疆农业大学动物医学院,乌鲁木齐,830052

收稿日期:2008-04-25 修回日期:2008-04-25 出版日期:2008-04-25 发布日期:2008-04-25

Comparison between Antibody Titers of Single-dilution Indirect ELISA and End-point Neutralizing Test of Bovine Rotavirus Vaccines

JIAN Zi-jian;CAO Zhu-ting;MA Su-zhen;GAO Wen-bin;ZHANG Xiao-hong;WANG Xiao-ping;YUAN Jiang-ling

Received:2008-04-25 Revised:2008-04-25 Published:2008-04-25 Online:2008-04-25

摘要/Abstract

摘要： 用NCDV标准毒株接种恒河猴肾细胞(MA-104),制备病毒抗原液,制成油乳佐剂灭活疫苗进行3月龄以内的犊牛田间免疫试验.结果表明,免疫组的OD492 nm平均值为0.6,免疫40 d后达到峰值1.5,免疫后60、90 d仍维持较高的抗体效价,OD492 nm平均值分别是1.4和0.9.中和抗体效价在二次免疫时、二次免疫40、60、90 d平均值分别为56.668、413.025、314.9、104.64.实验评价OD492 nm值和血清中和抗体效价关系,相关性很大,并证明这种疫苗对预防轮状病毒引起犊牛腹泻是安全有效的.

简子健;曹竹亭;马素贞;高文斌;张晓红;王晓萍;袁江玲. 单稀释法间接ELISA和终点法中和试验检测犊牛轮状病毒疫苗抗体效价比较[J]. .

JIAN Zi-jian;CAO Zhu-ting;MA Su-zhen;GAO Wen-bin;ZHANG Xiao-hong;WANG Xiao-ping;YUAN Jiang-ling. Comparison between Antibody Titers of Single-dilution Indirect ELISA and End-point Neutralizing Test of Bovine Rotavirus Vaccines[J]. .