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新疆小芦苇青贮中乳酸杆菌的分离鉴定

张想峰;艾尼瓦尔·艾山;买热木尼沙·吾甫尔;闫京阳;于苏甫·热西提   

  1. 新疆农业大学动物营养实验室,乌鲁木齐,830052;巴音郭楞蒙古自治州动物疾病控制与诊断中心,新疆库尔勒,841000;塔城地区畜牧科技研究推广中心,新疆塔城,834700
  • 收稿日期:2009-06-25 修回日期:2009-06-25 出版日期:2009-06-25 发布日期:2009-06-25

Isolation and Identification of Lactobacillus from Xinjiang Small Reed Silage

ZHANG Xiang-feng;Ainiwaer·Aishan;Mairemunisha·Wufuer;YAN Jing-yang;Yusufu·Rexiti   

  • Received:2009-06-25 Revised:2009-06-25 Published:2009-06-25 Online:2009-06-25

摘要: [目的]筛选新疆小芦苇青贮中优良的乳酸杆菌.[方法]研究将从自制的新疆小芦苇青贮中分离出乳酸杆菌进行鉴定,对鉴定出的菌株进行产酸试验,确定新疆小芦苇青贮中的优势菌株.[结果]试验共分离得到了11株乳酸杆菌,根据<伯杰氏细菌鉴定手册>(第八版)中的种属表述结果,经鉴定2株(Hz2-1和Hz2-2)为戊糖乳杆菌(L. pentosus),2株(Hz3-1和Hz3-2)为植物乳杆菌(L.plantarum),2株(Zar2-8和Zar2-9)为牛粪乳杆菌(L.dung),2株(Zar8-3和Zar8-4)为短乳杆菌(L. brevis),3株(Gsr1-1、Hz1-1和Zar9-4)为德氏乳杆菌保加利亚亚种(L.delbrueckii subsp.bulgaricus).[结论]从不同菌株发酵产酸试验的结果来看,发现Hz2-1,Hz3-1具有较强的产酸能力,pH最低可达3.50,是新疆小芦苇青贮发酵过程中的主要产酸菌株.

Abstract: [Objective]Xinjiang small reed distributed widely and which is good forage resources of ruminants. [Method]This study will isolate and identify Lactobacillus from the self-regulating Xinjiang small reed silage. The trains were acidogenic tests that identified the advantages trains of Xinjiang small reed silage. 11 strains of Lactobacillus were isolated from the self-regulating reed silage of different origin. According to the criteria and description of Bergey's manual of systematic bacteriology(The eighth edition),which include two strains Lactobacillus pentosus(Hz2-1,Hz2-2), two strains Lactobacillus plantarum(Hz3-1,Hz3-2), two strains lactobacillus dung(Zar2-8,Zar2-9), two strains Lactobacillus brevis(Zar8-3,Zar8-4) , three strains Lactobacillus delbrueckii subsp.bulgaricus (Gsr1-1,Hz1-1,Zar9-4) were identified.Based on a strain for the fermentation acid test, it was found that a strong acid production capacity were observed from HZ2-1, HZ3-1,the pH up the minimum could reach 3.50,which was the main strains from fermentation process of Xinjiang small reed.


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
地址:乌鲁木齐市南昌路403号新疆农业科学院
邮编:830091
电话:0991-4502046
E-mail:xjnykx-h@xaas.ac.cn


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