Abstract: [Objective and Method]With the ovary, style, filament of Jinkangka lily as the explant,the effects of different sterilizing time and mediums on the explant tissue culture process were studied. [Result]Light green callus tissue appear when the explants is cultrured for about 20 d.That is , different concentrations of NAA and BA have great impact on the callus induction. Hormone concentration of 0.5 mg/L NAA and 1.5 mg/L BA MS medium have the best impact on promoting the ovary swelling and callus induction formation.[Conclusion]The results showed that the effects of different sterilizing time on the contamination rate of the explant were different and they all showed downtrend with the prolonging of the sterilizing time.There are certain differences in the formation of callus ability among the Jinkangka lily ovary, style and filament. It is easier to form callus and have higher survival rates when we use the ovary as explants than use style and filament tissue. The explants were enlarged when it is cultured on the callus induced culture medium for about two weeks.