新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析

新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析

詹彬;安沙舟;杨丽娟;李莲瑞;马春晖

新疆农业大学草业与环境科学学院,乌鲁木齐,830052;塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300;新疆农业大学草业与环境科学学院,乌鲁木齐,830052;塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔,843300

收稿日期:2009-06-25 修回日期:2009-06-25 出版日期:2009-06-25 发布日期:2009-06-25

Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus

ZHAN Bin;AN Sha-Zhou;YANG Li-Juan;LI Lian-Rui;MA Chun-Hui

Received:2009-06-25 Revised:2009-06-25 Published:2009-06-25 Online:2009-06-25

摘要/Abstract

摘要： [目的]从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物.[方法]以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域.[结果]序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15;.[结论]得到新疆沙冬青的抗冻蛋白的保守区域.

Abstract: [Objective]A pair of primers were designed according to the three antifreezing proteins (AFP) gene of Ammopiptanthus mongolicus (Genbank accession number is AY590122, AY843521, and AY843522, respectively). [Method]Total RNA of Ammopiptanthus nanus was extracted using Trizol, which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.[Result]The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity (98.15;) to the AFP gene of Ammopiptanthus mongolicus.[Conclusion]The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus

詹彬;安沙舟;杨丽娟;李莲瑞;马春晖. 新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析[J]. .

ZHAN Bin;AN Sha-Zhou;YANG Li-Juan;LI Lian-Rui;MA Chun-Hui. Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus[J]. .