Abstract: [Objective and Method]Through the screening and orthogonal design experiments of the carbon, nitrogen and trace elements, [Result]the most suitable components of medium for strain's fermentation are found to be as follows (g/L): sucrose 60, peptone 30, NaCl 30, K_2HPO_4 2, MgSO_4 1, FeSO_4 0.02, MnSO_4 0.01. The research on fermental condition indicate that the optimal initial pH of fermentation is 5.5, the optimal temperature is 35℃,the optimal volume of liquid medium is 10 mL in 250 mL conical bottle. [Conclusion]The strain TS-Ⅱ's living barteria has reached 6.58×10~(10) CFU/mL after the optimization of the components of medium and cultivating condition.