Abstract: [Objective]The research aimed to obtain HKD2 domain in PLD gene and construct its antisense plant expression vector,so as to study the effect of PLDα gene on the storage property,[Method]total RNA was extracted from the mature fruit of strawberry,then cDNA was amplified by reverse transcriptase polymerase chain reaction(RT-PCR) and to clone T-A into pMD19-T simple vector. The positive clones were sent to sequence. [Result]After confirming its validity,the PLDα gene fragment was inverted into plant expression vector pBI121 to construct antisense expression vector pBI121-PLD.[Conclusion]Then the recombinant plasmid was transferred into Agrobacterium tumerfaciens LBA4404 by freeze-thaw method to further lay the base for extending the strawberry's storage.