Abstract: [Objective and Method]In this experiment,the upland cotton resistant parent Liaomian 18 and sensitive parent Junmian 1 and their F_2 generation were used for identification of the molecular markers linked to disease-resistance gene of cotton by SRAP-BSA,[Result]88 pairs of primers were used to amplify the parents gene and two gene pools. When 3 pairs of primers were found in parents and two pools,three pairs of primers were screened from 88 paris of SRAP primers.[Conclusion]The fragment existed in the resistant plants and F population,they were indicated that the SRAP marker were closely linked to disease-resistance gene of cotton.