Abstract: [Objective] This study aims to understand the genetic rules of main selection index of three lines hybrids of Xinjiang cotton with genetic effect analysis to screen high heterosis hybrid combination.[Method] Forty-two crosses were made between 7 sterile lines and 6 restorer lines by using 7 × 6NC Ⅱ cross design.The yield,lint percentage and fiber quality of F1 generation were used to carry out statistical analysis of genetic variance and combining ability.[Result] Yield was affected by both dominant and additive effects,and may be affected by overdominance effects.Lint percentage,length,micronaire and strength were predominantly affected by additive effect and party by partial dominance effect.In the sterile lines,3-248A showed the highest general combining ability,2-20A and 6-89A showed higher general combining ability,but other restorer lines showed low or negative general combing ability.In the restorer lines,12-278R showed the highest general combining ability,8-420R and 9-186R showed higher general combining ability,but other restorer lines showed low or negative general combing ability.In cross combinations,2 × 9 showed the highest specific combining ability,6 × 12,4 × 9,3 × 8 and 6 × 8 showed higher specific combining ability,exhibiting 23.33;,16.67;,15;,10.83; and 5; higher yield than that of the control,respectively,but other combinations showed low or negative specific combining ability.[Conclusion] Filter out the high advantages of hybrid 2 × 9,exhibiting 23.33; higher yield than that of control respectively,lint percentage and fiber quality of F1 generation made between 7 sterile lines and 6 restorer lines with 7 ×6NC Ⅱ cross design.