Abstract: [Objective] Sequence Tagged Site(STS)is a short DNA sequence,which appears only once on its biological chromosome and is suitable for a landmark of crop genome.[Method]500 STS-SSR primers of sunflower were synthesized randomly and were used to screen the samples of Xinjiang edible sunflower and oil sunflower inbred lines for finding the polymorphism.[Result]All of them 62 markers showed stable polymorphism among those 136 sunflowers with clear,stable and easy to read characteristics,which accounted for 12.4;of the number of selected primers.136 Xinjiang sunflower inbred populations could be divided into 2 groups according to the change of △K and be clustered into 2 groups at the 0.65 similarity level.In the 73 oil sunflower inbred lines,70;inbred lines were assigned to first groups which meant the oil sunflower inbred lines were mainly classified in the first group.Among 30 maintainer lines of oil sunflower lines only 4 maintainer lines were assigned to second groups,the rest were in group I.In the 43 restorer line of oil sunflower,25 restorer lines were allocated to first large groups,and the rest were allocated to other groups,which indicated that the genetic diversity of restorer line was more abundant than maintainer lines.In the 55 edible sunflower inbred lines,only 21;of them were divided into first groups which indicated that edible sunflower inbred lines belonged to the second and the third groups.[Conclusion]This study showed that the genetic diversity of edible sunflower was greater than that of oil sunflower,and some genes between the edible sunflower and oil sunflower materials were crossed and penetrated.The results of this study have provided theoretical support for the construction of the core collection and the rational utilization of resources in the future.