Abstract: [Objective] During tissue culture of German irises, Endophytic bacteria pollution is the key problem of restricting them for rapid propagation factory nursery.For that reason, this project aims to segregate and identify endophytic bacteria that might lead to tissue culture explants pollution, and the inhibition of 6 kinds of commonly used agents on the strain effect will be compared so as to select the best preventive agent.That will provide a scientific basis for effective control of endophytic bacteria pollution during its rapid propagation.[Method]The strain (115325) has been segregated and purified using NA medium and the endophytic bacteria were been classified and identified by morphology observation, physiological and biochemical test, and 16S rDNA sequence homology analysis.Inhibitory effects of 6 kinds of commonly used chemicals on the tested strains were determined by the method of paper disc bacteriostatic circle.[Result]The strain (115325) of morphology characteristics and physiological and biochemical indicators were basically the same as Staphylococcus epidermidis.The results of 16S rDNA sequence homology analysis showed that the strain and saccharolyticus(L37602) had mostly close relation in the evolution, belonging to the same phylogenetic branch.And S.staphylococcus epidermidis had the highest homology, at 99.86;.Indoor medicament screening results showed that the cephalosporins and gentamicin had the strongest inhibitory effect on strains.[Conclusion]The comprehensive analysis results of morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence, 115325 strains have been identified as Staphylococcus epidermidis.Cephalosporin and gentamicin can be used as the preferred agents to inhibit endophytic bacterial contamination of the German iris plantlets.