Cloning and bioinformatics analysis of VvGST1 from Munage tab.grapes

【Objective】 To clone and study the sequence characteristics of the glutathione-S-transferase, (GST) gene from the Vitis vinifera L.cv ‘Munage’ table grapes in the hope of laying a foundation for the disease resistance and function of this gene in grape fruit. 【Methods】 Full-length VvGST1 gene cDNA was isolated from grape fruit according to the existed ESTs sequence,and 3' and 5' end of the RACE primers were designed by using RT-PCR and RACE. 【Results】 A variety of bioinformatics analysis methods were used to predict and analyze the gene, and the results showed that the ORF (Open Reading Frame) length was 719 bp, which encoded a 221 amino acid polypeptide.The VvGST1 gene was forecasted and the relative molecular mass of encoding the protein was 25.55 kDa, the theoretical isoelectric point was 6.32, the atomic composition was C₁₁₇₈H₁₇₉₉N₂₉₃O₃₂₁S₁₁.Its instability coefficient was 39.22, showing it was astable hydrophilic protein without signal peptide or transmembrane structure.It might exist in the cell matrix and was a typical matrix protein.Through analyzing the evolutionary tree, the amino acid sequence of VvGST1 was clustered with cotton, peach, prunus, cherry, plum, chestnut and other plants, among which it was closely related to cotton. 【Conclusion】 The full-length coding region of VvGST1 gene in Vitis vinifera L.cv ‘Munage’ table grapes is successfully obtained,and the structural characteristics of the sequence are confirmed.