Transcriptome analysis of callus at rooting stage in tissue culture of vitis amurensis 'shuangyou'

【Objective】 In order to explore the molecular mechanism of rooting of Vitis amurensis and provide theoretical data and reference basis for rooting in production and reproduction of Vitis amurensis. 【Methods】 From the tissue culture to the rooting stage of Vitis amurensis ‘Shuangyou’, the two groups with large differences in the number of roots were treated with different concentrations of IBA (0.1, 0.3 and 0.5 mg/L), NAA (0.1, 0.3 and 0.5 mg/L) and sucrose (15, 25 and 35 g/L),and the transcriptome of the callus rooting at the base was sequenced. 【Results】 A total of 284.40 Gb high-quality sequences were obtained from 6 samples, and 89.15%-90.3% of Clean Reads were compared to the reference genome. Compared with S1, there were 537 differentially expressed genes significantly up-regulated in S6 and 277 differentially expressed genes significantly down regulated in S1, a total of 814 differentially expressed genes. A total of 805 differentially expressed genes were annotated into 21 categories of COG, mainly including unknown function, intracellular transport and signal transduction mechanism. GO enrichment analysis showed that the differentially expressed genes were mainly enriched in polysaccharide binding, kinesin complex and auxin activated signal pathway. KEGG enrichment analysis showed that the differentially expressed genes were enriched in phenylpropane biosynthesis, flavonoid biosynthesis and plant hormone signal transduction. The expression levels of 16 differentially expressed genes in plant hormone signal transduction pathway were analyzed. The 11 differentially expressed genes in auxin pathway were up-regulated, and the 5 genes with large differences were IAA4, IAA22, IAA27, LAX3 and A-ARR genes. The transcriptome data were verified by qRT-PCR. 【Conclusion】 By sequencing the callus of 'Shuangyou' grape tissue culture plantlets induced by different concentrations of IBA, NAA and sucrose, a relatively complete transcriptome data is obtained, and its function annotation, enrichment analysis and hormone related gene expression analysis are carried out.