Cloning and Functional Analysis of Cytochrome P450 Superfamily Gene GhCYP85A2-1 in Upland Cotton

【Objective】 The principal objective is to investigate the biological function of CYP85A subfamily genes of the cytochrome P450 superfamily in the development of cotton plant height in the hope of providing a scientific basis for molecular breeding of upland cotton plants. 【Method】 A CYP85A family gene GhCYP85A2-1 was cloned from upland cotton by homologous cloning. Tobacco rattle virus (TRV) induced gene silencing technology (Virus-induced gene silencing, VIGS) was used to construct GhCYP85A2-1 gene silencing vector, total RNA was extracted, and gene expression of GhCYP85A2-1 in infecting cotton seedlings was investigated by qRT-PCR. 【Result】 The study found that the height of GhCYP85A2-1 gene silenced plants was significantly reduced, and so was the gene expression. 【Conclusion】 GhCYP85A2-1 gene plays an important regulatory role in the development of cotton plant height.