Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus

ObjectiveA pair of primers were designed according to the three antifreezing proteins (AFP) gene of Ammopiptanthus mongolicus (Genbank accession number is AY590122, AY843521, and AY843522, respectively). MethodTotal RNA of Ammopiptanthus nanus was extracted using Trizol, which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.ResultThe result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity (98.15;) to the AFP gene of Ammopiptanthus mongolicus.ConclusionThe above result could also set the base for the complete AFP gene of Ammopiptanthus nanus