Cloning and Expression Analysis of HcDNA pol λ Gene from Halostachys caspica under Salt Stress

Objective The expression of DNA damage repair gene under salt stress is helpful to reveal the relationship between DNA damage repair and salt tolerance of halophyte.MethodAccording to the transcriptome of Halostachys caspica under salt stress,a DNA damage repair gene DNA polymerase lambda(HcDNA pol λ)was cloned from Halostachys caspica by RACE.ResultSequence analysis indicated that HcDNA pol λ contained an open reading frame of 1,335 bp,which encodes 444 amino acids.Conserved domain analysis showed that HcDNA pol λ was the number of DNA polymerase X family,and phylogenetic tree analysis indicated that HcDNA pol λ was an independent branch,which was an stable hydrophilic proteins sub-celled nuclear.Real time fluorescent quantitative PCR analysis showed that the expression of HcDNA pol λ of assimilating branches gradually up-regulated with the increase of NaCl concentration.At 300 mmol/L NaCl treatments,the expression levels of HcDNA pol λ were increased 3 fold and 5 fold for 7 days and for 14 days,respectively.Finally,at 700 mmol/L NaCl treatment for 14 days,the expression levels of HcDNA pol λ increased 20 fold and reached the peak.ConclusionThe expression of HcDNA pol λ gene could be induced by salt stress.