Study on the Induction and Proliferation of Somatic Embryogenesis from the Mature and Immature Zygote Embryos of Picea schrenkiana

Objective To study the induction and proliferation of somatic embryogenesis from the mature and immature zygote embryos of Picea schrenkiana,which might have great significance for the understanding of the mechanism of embryonic development and the establishment of rapid propagation system.MethodImmature embryo and mature embryo were used as explants to induce culture in DCR medium with different growth regulator concentrations.The induction rates of zygotic embryos in different growth stages and its proliferation were studied using immature zygotic embryos with endosperm,immature zygotic embryos without endosperm and mature embryos as explants.Result(1)When DCR medium added with different concentrations of 2,4-D and 6-BA was used to induce mature zygotic embryos,the combination of 20 μM 2,4-D and 5 μM 6-BA was the best and somatic embryogenesis induction rate was the highest,up to 55;.(2)The induction rate of three zygotic embryos of somatic embryogenesis collected in different periods showed that the immature zygotic embryos with endosperm(June)was up to 32.6;,the mature zygotic embryos(September)was up to 60.6;,Immature embryo without endosperm(July)was 83.1;,which indicated that July was the best picking period of P.schrenkiana cones.(3)The somatic embryogenesis in the five different proliferation medium showed the the proliferation rate was significant(P< 0.05).The proliferation results of somatic embryogenesis in different proliferation medium showed that the effect of BM2 and SH were better than the MSG,1/2DCR,DCR,and the comprehensive proliferation effect of SH was the best.The proliferation rate was 4.55 g and the rate was up 600;.)(4)The five different somatic embryogenesis in SH proliferation medium showed that the difference between somatic embryogenesis was not significant(P > 0.05).ConclusionThis study has established the somatic embryogenesis technology platform of P.schrenkiana for the first time,which has provided a scientific basis for the genetic improvement of germplasm and shortened the breeding cycle of P.Schrenkiana.