Molecular detection of Jujube Badnavirus WS in Southern Xinjiang

Objective In order to clarify the infection dynamics of Jujube Badnavirus WS in the field, and to explore the key time nodes for disease control.

Methods A sequence-specific primer was designed based on the full genome sequence of Jujube Badnavirus WS. Three seriously affected jujube orchards were identified in the disease occurrence area, and the established molecular detection technique was used to track and detect the infection of Jujube Badnavirus WS in the field.

Results The lowest concentration of total DNA for the detection of Jujube Baculovirus WS is 4.526 pg/μL, and the virus can be detected in the latent stage of jujube leaves. Through the tracking detection and analysis of the infection dynamics in the field, it was clear that the mid-to-late May was the key time node to control the jujube virus disease.

Conclusion The established molecular detection technique is practical and easy to operate, and can accurately and quickly track and detect the field infection of Jujube Badnavirus WS. It is preliminarily confirmed that the positive rate of infected jujube leaves increases with the increase of month, and the latent disease occurs in July and reaches the peak in August. Jujube Badnavirus WS was detected in all jujube leaves with symptoms. Jujube Badnavirus WS has a direct correlation with the disease of jujube mosaic leaves and fruit deformities, and is most likely the major pathogen of jujube virus disease.