Optimization of extraction process and purification process of active components from Pleurospermum lindleyanum by response surface methodology

Objective To optimize the extraction and purification process of active components from in Pleurospermum lindleyanum.

Methods HPLC method was used to determine the content of active ingredients in Pleurospermum lindleyanum. By conducting static and dynamic experiments, the effects of resin type, sample concentration, sample flow rate, maximum sample volume, elution water volume, eluent concentration, elution flow rate and elution volume were investigated to determine the optimal purification process.

Results The results showed that the optimal extraction process for active ingredients of Pleurospermum lindleyanum was as follows: ethanol concentration of 30%, solid-liquid ratio of 1∶8 (g/mL), extraction time of 2 hours, and two extractions. The yield of active ingredients reached 58.60 mg/g. Among the 7 types of macroporous adsorption resins compared, H103 type showed better purification effect. The optimal purification conditions were as follows: sample concentration of 2.5 mg/mL, sample loading of 6.35 BV at a flow rate of 1 BV/h, washing with elution liquid volume of 3.17 BV, and eluting with elution liquid consisting of 80% ethanol at a flow rate of 3 BV/h for a volume of 2.54 BV. Under these conditions, the purity of active components in Pleurospermum lindleyanum reached 40.03%, with a transfer rate of 76.26%.

Conclusion The measurement method is accurate and reliable. The study demonstrates that the extraction and purification process is stable, reasonable, and feasible in improving the content of active components in Pleurospermum lindleyanum, laying a foundation for further development and utilization.