Objective Study on the resistance at the seedling stage to stripe rust races CYR32, CYR33 and CYR34 in spring wheat varieties (lines) in Xinjiang and its resistance genes distribution.
Methods Seedling resistance was evaluated by inoculating 71 Xinjiang spring wheat varieties with CYR32, CYR33, and CYR34 races during the seedling stage. Subsequently, molecular markers specific to 7 stripe rust resistance genes (Yr5, Yr9, Yr10, Yr15, Yr18, Yr26, and Yr80) were employed to detect the presence of these genes in the test materials.
Results Among 71 materials, a total of 63 materials showed seedling resistance, accounting for 88.73%, and 38, 43 and 47 materials showed resistance to CYR32, CYR33 and CYR34 races, respectively, accounting for 53.52%, 60.56% and 66.20%, 19 wheat varieties (26.76%) showed seedling resistance to all CYR32, CYR33 and CYR34. Molecular marker detection results indicated that 17 varieties (lines) may carry resistance genes, of which 13 and 5 materials may carry Yr9 and Yr80, respectively. Only the variety Hechun-97 might carry both Yr9 and Yr80. None of the tested varieties (lines) were detected with Yr5, Yr10, Yr15, Yr18, and Yr26.
Conclusion The highest frequency of seedling resistance to CYR34 was observed in the material, Only a small amount of Yr9 and Yr80 was detected in all the materials, which may have potential unknown stripe rust resistance genes. In this study, we clarified the resistance at seedling stage and the distribution of resistance genes in 71 Xinjiang spring wheat varieties (lines), providing a basis for breeding durable resistant wheat varieties against stripe rust.
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