Objective To clone the full-length transcription factors VvWRKY11 and VvWRKY75 in Red Earth Grape berries and study their sequence characteristics in the hope of laying the foundation for the study of the role and function of these transcription factors in grape berries' disease resistance.
Methods Based on the transcriptome sequencing data of pre-harvest SA sprayed grapes, we screened and cloned two WRKY transcription factors, VvWRKY11 and VvWRKY75, which were significantly differentially expressed in SA-induced fruit in response to Staphylococcus griseus, and then further analyzed the sequences of VvWRKY11 and VvWRKY75 by bioinformatics methods.
Results The full length of VvWRKY11 and VvWRKY75 gene sequences were 1 075 and 965 bp, respectively, with open reading frames of 894 and 570 bp, encoding 297 and 189 amino acids, respectively, and with termination codons TGA and TAA, respectively, and with predicted relative molecular weights of 32.36 and 21.26 kD, respectively, and theoretical isoelectric points of 9.73 and 9.13, both of which were unstable hydrophilic proteins. The secondary structures of both grape VvWRKY11 and VvWRKY75 proteins consist mainly of irregularly coiled and α-helices, and the promoters of both genes contain hormone-responsive elements such as the W-box, methyl jasmonate, salicylic acid and abscisic acid.
Conclusion The full-length coding sequences of the Red Earth Grape transcription factors VvWRKY11 and VvWRKY75 genes are obtained, and the structural features of the sequences are explored.
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