Optimization of SE medium of a saline-alkali green algae

【Objective】 Optimization of SE medium of a saline-alkali green algae,the purpose of this study is to provide a theoretical basis for the research on the molecular biology and ecological environment protection of saline-alkali green alga as well as fishery bait resources. 【Methods】 A saline-alkali green alga isolated and purified from East Lake of Tarim University is screened for culture medium, morphological observation and molecular identification.Investigate the effects of CaCl₂·2H₂O, KH₂PO₄, NaCl, NaNO₃, MgSO₄·7H₂O and pH on its growth, and conduct phylogenetic analysis. 【Results】 The morphological characteristics showed that the algae is ellipse.The optimum concentration of CaCl₂·2H₂O is 0.050 g/L, the soluble sugar is 24.34%, the soluble protein is 16.55%, and the total chlorophyll is 2.000 g/L.The optimum concentration of KH₂PO₄ is 0.175 g/L, the soluble sugar is 10.53%, the soluble protein is 7.7%, and the total chlorophyll is 3.607 g/L.The optimum concentration of NaCl is 0.100 g/L, the soluble sugar is 11.82%, the soluble protein is 8.57%, and the total chlorophyll is 3.385 g/L.The optimum concentration of NaNO₃ is 0.250 g/L, the soluble sugar is 10.64%, the soluble protein is 6.10%, and the total chlorophyll is 2.731 g/L.The optimum concentration of MgSO₄·7H₂O is 0.375 g/L, the soluble sugar is 12.32%, the soluble protein is 9.9%, and the total chlorophyll is 1.790 g/L.The optimum pH concentration is 9.0, the soluble sugar is 16.57%, the soluble protein is 9.46%, and the total chlorophyll is 1.985 g/L.The results showed that the sequence of No.1 algae is closely related to the novel Pseudochlorococcum sp. 【Conclusion】 This study determined the classification status of the saline No.1 and the concentration of CaCl₂·2H₂O, KH₂PO₄, NaCl and NaNO₃ after SE medium optimization was 0.050 g/L, 0.175 g/L, 0.100 g/L, 0.250 g/L, respectively. The concentration of MgSO₄·7H₂O is 0.375 g/L and the pH is 9.0.