Application Study on the Adjuvant Jijian as Synergist for Cotton Verticillium Disease Control

【Objective】 To provide technical support for the reduction and efficiency of chemical pesticides for cotton Verticillium disease control by comprehensively evaluating the fungicides and application methods of Jijian against Verticillium dahliae V991 using optimizing the composition of fungicides, elicitors and auxiliaries commonly used in production. 【Methods】 In order to screen the fungicide with significantly synergistic control effect on V991 mycelia, the mycelial growth rate experiment was carried out by the combination of the combination of carbendazim / Jijian, ethylicin / Jijian and Bacillus subtilis/ Jijian. Through the potted Verticillium wilt control experiment, the application measures and methods of inducer, pesticide/Jijian were determined. 【Results】 Jijian could be used as synergistic agent to increase the bacteriostatic rate of carbendazim and etallicin for controlling V991 mycelia growth. Adding Jijian, the inhibition rate of 50 mg/kg carbendazim increased from 19.68% to 35.49%, and 14.83% to 26.29% respectively, and EC₅₀ of carbendazim and ethylicin also decreased to 0.104 mg/kg from 1.707 mg/kg, and 0.824 mg/kg from 1.249 mg/kg separately, and the pesticide dosage also decreased 16.41 times and 1.52 times accordingly. The study on potted cotton Vercillium wilt showed that the best control effect was determined by spraying amino-oligosaccharide before or after inoculating pathogen sequentially equipped with the combination of carbendazim / Jijian-ethylicin / Jijian, and the control efficacy was still as high as 69.27% and 62.70% 28 days later. 【Conclusion】 Jijian can be used as synergistic agent of carbendazim and ethylicin for the cotton Verticillium disease control which dramatically reduces the pesticides dosages, and the combination of carbendazim / Jijian-ethylicin / Jijian employed in turn had the best control effect on potted cotton Verticillium wilt with spraying amino-oligosaccharides inducer 24h before or after inoculating pathogen.