Segregation Distortion of Progeny of Distant Hybridization of Brassica juncea Introduced into Chinese Cabbage Genome

【Objective】 Introduce the A genome of Chinese cabbage into core mustard by distant hybridization, and use high-resolution melting curve (HRM) combined with InDel marker-assisted selection technology to screen out the most preserved cabbage Crossing offspring, with a view to improving the nodularity of core mustard and enriching the germplasm resources of core mustard. 【Methods】 Using immature embryo culture technology to assist 6 servings of core mustard and 2 servings of Chinese cabbage for distant hybridization and backcrossing, 20 InDel markers were selected to track all chromosomes of the Chinese cabbage A genome, and screened from the 192 BC₁ single plants, the single plants with a high percentage of Chinese cabbage A genome markers. Furthermore, through field investigations, single plants with similar heading to cabbage were selected for further research. 【Results】 Nine interspecific hybrid combinations and 14 BC₁ populations were obtained by remote hybridization combined with immature embryo culture. 203 HRM-InDel markers that can be uniformly distributed throughout the genome to distinguish between cabbage and core mustard were screened and obtained; 20 InDel markers were used to track the cabbage genes of 192 BC₁ single plants. The mark content exceeds 90%, which accumulates materials for further research work. 【Conclusion】 Immature embryo culture technology can improve the seed setting rate of the backcross of Chinese cabbage and core mustard, and can speed up the breeding process; the combination and single plant with a high proportion of Chinese cabbage genome were selected; the study found that the isolated population was significantly biased towards transmission Cabbage gene.