Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes

【Objective】 More convenient and efficient identification techniques need to be explored to improve the identification rate.which can be used for rapid and accurate identification of Cicadellidae and provide basic data for the identification and prevention of Cicadellidae in this area. 【Methods】 In this research DNA bar-coding technology was used to obtain DNA bar-code of Cicadellidae, during which COI, Cytb, ITS2 genes were selected, PCR amplification was applied with universal primers and sequenced directly. Bioinformatics analysis software was used to compare and analyze the similarity of target gene sequences. Phylogenetic tree was constructed to analyze genetic and evolutionary relationship. 【Results】 19 mtDNA sequences of COI,Cytb and ITS2 genes were acquired as DNA barcodes of Cicadellidae, including six COI genes, seven Cytb genes and 6 Cytb genes; Among them, one was a new COI sequence, five Cytb sequences and 6 ITS2 sequences, all of which were the first to be reported in this region. 【Conclusion】 Nineteen DNA bar codes of Cicadellidae are obtained, the rapid and accurate identification of 4 species of farmland’s Cicadellidae in the southern foothills of Pamirs Plateau came true.