Construction of the Fingerprints of 19 Self-bred Grape (Vitis vinfera) Varieties (Lines)

【Objective】 To construct the fingerprints of 19 grape varieties (lines) by SSR technology in the hope of providing basis for seedling certification, variety registration and new plant variety protection. 【Method】 In this project, 19 grape varieties (lines) were used as test materials, 16 pairs of SSR labeled silver primers were used for PCR amplification and capillary electrophoresis to obtain the fingerprint bands, construct the fingerprint map, and select the primer combinations that could distinguish the 19 varieties (lines), encode the alleles and establish the germplasm molecular identity card. 【Result】 In this study, VRZAG67, VVS2, VVMD7, VCHR4a and VVMD5 were selected from 16 pairs of SSR markers, and 5 pairs of SSR markers with relatively high amount of polymorphism information were constructed. The fingerprints of varieties (lines) were constructed, and their assigned values were coded to form an identification code. 【Conclusion】 The fingerprints and molecular ID cards constructed can identify and distinguish 19 varieties (lines), but because there are many grape germplasms, if we continue to distinguish more varieties, we needed to increase the number of SSR primers.