Study on HPLC Fingerprint of Hymenoleana nana

【Objective】 To establish the HPLC Fingerprint of Hymenoleana nana and provide a scientific basis for the quality control, the development and utilization of medicinal plant resources. 【Method】 HPLC was performed using a WondaCract ODS-2 C₁₈ column(4.6 mm×250 mm,5 μm), with of mobile phase acetonitrile: 0.1% formic acid water gradient elution, flow rate 1.0 mL/min, column temperature 30℃, detection wavelength 283 nm. The fingerprints of 10 batches of medicinal materials were determined and the fingerprints were analyzed by cluster analysis, principal component analysis and least squares-discriminant analysis using the Similarity Evaluation System for Chromatographic Fingerprint of TCM and statistical software. 【Result】 The chromatographic evaluation software calibrated 13 common chromatograms, and the similarity of 7 fingerprints in 10 batches was >0.9. 【Conclusion】 The established method has good precision, stability and repeatability, which lays a foundation for the total quality control of the medicinal materials of Hymenoleana nana.