Effects of Two Types of Expression Vectors on Biological Characteristics of Acidovorax citrulli

【Objective】 This project aims to analyze the effects of two types of expression vectors on the biological characteristics ofAcidovorax citrulli in the hope of providing a theoretical reference for the study of gene function.【Method】The wild type FC440 strains of watermelon acid bacteria were transferred into the expression vectors pBBR1MCS-5 and pHC60 respectively. Then the extracellular cellulase activity, extracellular protease activity, extracellular polysaccharide content, mobility, pathogenicity and hypersensitive reaction were obtained and the effects of growth rate and copper resistance on the biological characteristics of the transformed strain were analyzed.【Result】The results showed that vector pBBR1MCS-5 had no significant effect on the biological characteristics ofAcidovorax citrulli, but vector pHC60 significantly inhibited the growth rate and motility of the strain. 【Conclusion】During the study of gene function, the expression vector itself may affect some biological characteristics ofAcidovorax citrulli. It is necessary to choose an appropriate expression vector while exploring the function of a gene.