Effects of sulfur dioxide on sugar content and sugar metabolism pathway in Munage grape

doi:10.6048/j.issn.1001-4330.2023.05.021

Abstract

Abstract:

【Objective】 To study the effect of sulfur dioxide (SO₂) on sugar content and sugar metabolism pathway in Munage grape fruit.【Methods】 The changes of sucrose, glucose and fructose contents in Munage grapes during storage were determined by high performance liquid chromatography (HPLC) and simultaneously, the expression differences of 20 genes related to glucose metabolism were evaluated.【Results】 SO₂ treatment significantly increased the glucose content from 0 to 10 days of storage.On the 60th day of storage, the contents of sucrose, glucose and fructose in the SO₂-treated grape were increased to varying degrees compared with that of the control group.SO₂ up-regulated cell wall invertase (CWINV), neutral/alkaline invertase (CIN), sucrose synthase (SuSy) and sucrose phosphate synthase1 (Sucrose phosphate synthase1, SPS1) gene expression level to maintaine high sugar content in grape during storage.Moreover, SO₂ activated the photosynthetic activity of grape by inducing the expression of hexokinase 2 (HxK2), fructose-bisphosphate Aldolase (FBA), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK).【Conclusion】 SO₂ can regulate the expression levels of related genes to modulate the glucose metabolism-related pathways in the postharvest storage of Munage grapes.

Key words: Munage grape; sulfur dioxide; high performance liquid chromatography; sugar metabolism

摘要：

【目的】研究二氧化硫(sulfur dioxide,SO₂)对木纳格葡萄果实中糖含量及糖代谢途径的影响。【方法】基于高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定木纳格葡萄果实贮藏过程中蔗糖、葡萄糖和果糖含量变化,分析20个与糖代谢有关基因的表达差异性。【结果】SO₂处理使得贮藏0~10 d时葡萄糖含量显著提高。贮藏第60 d,SO₂处理组果实蔗糖、葡萄糖和果糖含量较对照组有不同程度的提高。SO₂通过上调细胞壁转化酶(Cell wall invertase,CWINV)、中性/碱性转化酶(Neutral/Alkaline invertase,CIN)、蔗糖合成酶(Sucrose synthase,SuSy)和蔗糖磷酸合成酶1(Sucrose phosphate synthase1,SPS1)基因的表达水平来维持葡萄果实的高糖含量。调控己糖激酶2(Hexokinase2,HxK2)、果糖-二磷酸羧醛酶(Fructose-bisphosphate Aldolase,FBA)、甘油醛-3-磷酸脱氢酶(Glyceraldehyde-3-phosphate dehydrogenase,GAPDH)和磷酸甘油酸激酶(Phosphoglycerate kinase, PGK)基因的表达水平,SO₂激活葡萄果实采后贮藏过程中的光合活性。【结论】SO₂可以调控相关基因的表达水平以调节木纳格葡萄采后贮藏中的糖代谢相关途径。

关键词: 木纳格葡萄, 二氧化硫, 高效液相色谱, 糖代谢

CLC Number:

S663.1

CHANG Xuehua, YAN Bowen, ZHAI Rongzhen, ZHANG Zheng, WU Bin, WEI Jia. Effects of sulfur dioxide on sugar content and sugar metabolism pathway in Munage grape[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1216-1225.

常雪花, 闫波雯, 翟荣臻, 张政, 吴斌, 魏佳. 二氧化硫对木纳格葡萄采后糖含量及糖代谢途径的影响[J]. 新疆农业科学, 2023, 60(5): 1216-1225.

Figures/Tables 7

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基因 Gene	登陆号 Login number	名称 Name	引物序列(5'-3') Primer sequences(5'-3')
VvCWINV	AY538262.1	CWINV-F	CCGATCTTGTCAACTGGACTCCTC
VvCWINV	AY538262.1	CWINV-R	TGAACCTGACCAGCAGCCATTG
VvHxK1	XM_002283572.4	HxK1-F	GCTGACGCCATGACTGTTGAG
VvHxK1	XM_002283572.4	HxK1-R	CACACGCAAGACACGGAAGTT
VvHxK2	XM_002283538.3	HxK2-F	CTTGCGGCTGCTGCTATCTTC
VvHxK2	XM_002283538.3	HxK2-R	CCCAACCAATTCTCTCAGTGTGTT
VvHxK3	XM_002274723.3	HxK3-F	AAGGAGTCGTGGAAGTGATGGTAA
VvHxK3	XM_002274723.3	HxK3-R	TGAAGAATGTGAAGCAGCAAGGAG
VvSuSy	XM_010657781.2	SuSy-F	TGGAAGAGCAGTCAGAGATGAAGA
VvSuSy	XM_010657781.2	SuSy-R	GTCAGCAATGTAGCGGTAGAGTTC
VvSPS1	NM_001280928.1	SPS1-F	CCTTGAATGTGCCAATGGTGCTA
VvSPS1	NM_001280928.1	SPS1-R	CTCCTCAGCCTCAATTCTCCTCAT
VvSPS3	XM_002271362.4	SPS3-F	GCGGCGGCGAAGATTGATT
VvSPS3	XM_002271362.4	SPS3-R	ACACTGGCATTGCTGTTGATAGA
VvFRK1	XM_002273540.4	FRK1-F	AATGGACGATTGGTGGAGTGAGA
VvFRK1	XM_002273540.4	FRK1-R	GGTCTTGACTAGGTTGTGGAGTGA
VvFRK2	XM_010651651.2	FRK2-F	AAGAAGGTGAGGACACGAAGGAA
VvFRK2	XM_010651651.2	FRK2-R	TGGTCTATAAGTCTGTTGGCTGGT
VvSPP1	XM_002269774.4	SPP1-F	AAAGTTGCCACGATTCCATCTCC
VvSPP1	XM_002269774.4	SPP1-R	GCGATCCATGATTCACCACGATAG
VvSPP2	XM_019223242.1	SPP2-F	GCTGCTATGGTGACAAACAAGGAA
VvSPP2	XM_019223242.1	SPP2-R	CAGTGCCGCTCATCTCCAGAT
VvCIN	XM_003632216.3	CIN-F	CCACGGCAGTTCCTCACCTT
VvCIN	XM_003632216.3	CIN-R	ACCCTGAATTGAATAGACCCTCCT
VvPGM	XM_010660101.2	PGM-F	AAGTTGGCATGGACTGGCATCTC
VvPGM	XM_010660101.2	PGM-R	TCAACACCAGCAACAGCTACACC
VvGPI	XM_002282738.3	GPI-F	CTCTCGTCTCGTCTCCGTCAA
VvGPI	XM_002282738.3	GPI-R	TGGCATCTCTTCGTGTCACTCA
VvFBA	XM_002283345.3	FBA-F	GCTTATTGCCAATGCTGCCTACA
VvFBA	XM_002283345.3	FBA-R	TGTGCCCTTGTCTACTTTGATTCC
VvGAPDH	XM_002263109.3	GAPDH-F	GTCAACGATCCATTCATCACCACT
VvGAPDH	XM_002263109.3	GAPDH-R	AGCAGCCTTGTCCTTGTCAGT
VvPGK	XM_019221715.1	PGK-F	GCCTATGGTATTCTTGATGGTTGGAT
VvPGK	XM_019221715.1	PGK-R	TTTCGCAGTTGCTTTGAGTTAGTCT
VvPGMM	XM_019219069.1	PGMM-F	GAGAAGCATCAGCGACTATCAG
VvPGMM	XM_019219069.1	PGMM-R	CCGTGTCCACAACCAATTCAT
Vvenolase	XM_002267055.2	enolase-F	TGTCACTTGCCGTGTGTAAAGC
Vvenolase	XM_002267055.2	enolase-R	AGCCACCTTCATCACCAACATTG
VvPK	XM_019221576.1	PK-F	ATCACTCTCACTGTCTTGTCTTGT
VvPK	XM_019221576.1	PK-R	AATGTCTTCCTTGTCCTTCTCTGT
VvActin	AB073011.1	Actin-F	GATTCTGGTGATGGTGTGAGT
VvActin	AB073011.1	Actin-R	GACAATTTCCCGTTCAGCAGT

基因 Gene	登陆号 Login number	名称 Name	引物序列(5'-3') Primer sequences(5'-3')
VvCWINV	AY538262.1	CWINV-F	CCGATCTTGTCAACTGGACTCCTC
VvCWINV	AY538262.1	CWINV-R	TGAACCTGACCAGCAGCCATTG
VvHxK1	XM_002283572.4	HxK1-F	GCTGACGCCATGACTGTTGAG
VvHxK1	XM_002283572.4	HxK1-R	CACACGCAAGACACGGAAGTT
VvHxK2	XM_002283538.3	HxK2-F	CTTGCGGCTGCTGCTATCTTC
VvHxK2	XM_002283538.3	HxK2-R	CCCAACCAATTCTCTCAGTGTGTT
VvHxK3	XM_002274723.3	HxK3-F	AAGGAGTCGTGGAAGTGATGGTAA
VvHxK3	XM_002274723.3	HxK3-R	TGAAGAATGTGAAGCAGCAAGGAG
VvSuSy	XM_010657781.2	SuSy-F	TGGAAGAGCAGTCAGAGATGAAGA
VvSuSy	XM_010657781.2	SuSy-R	GTCAGCAATGTAGCGGTAGAGTTC
VvSPS1	NM_001280928.1	SPS1-F	CCTTGAATGTGCCAATGGTGCTA
VvSPS1	NM_001280928.1	SPS1-R	CTCCTCAGCCTCAATTCTCCTCAT
VvSPS3	XM_002271362.4	SPS3-F	GCGGCGGCGAAGATTGATT
VvSPS3	XM_002271362.4	SPS3-R	ACACTGGCATTGCTGTTGATAGA
VvFRK1	XM_002273540.4	FRK1-F	AATGGACGATTGGTGGAGTGAGA
VvFRK1	XM_002273540.4	FRK1-R	GGTCTTGACTAGGTTGTGGAGTGA
VvFRK2	XM_010651651.2	FRK2-F	AAGAAGGTGAGGACACGAAGGAA
VvFRK2	XM_010651651.2	FRK2-R	TGGTCTATAAGTCTGTTGGCTGGT
VvSPP1	XM_002269774.4	SPP1-F	AAAGTTGCCACGATTCCATCTCC
VvSPP1	XM_002269774.4	SPP1-R	GCGATCCATGATTCACCACGATAG
VvSPP2	XM_019223242.1	SPP2-F	GCTGCTATGGTGACAAACAAGGAA
VvSPP2	XM_019223242.1	SPP2-R	CAGTGCCGCTCATCTCCAGAT
VvCIN	XM_003632216.3	CIN-F	CCACGGCAGTTCCTCACCTT
VvCIN	XM_003632216.3	CIN-R	ACCCTGAATTGAATAGACCCTCCT
VvPGM	XM_010660101.2	PGM-F	AAGTTGGCATGGACTGGCATCTC
VvPGM	XM_010660101.2	PGM-R	TCAACACCAGCAACAGCTACACC
VvGPI	XM_002282738.3	GPI-F	CTCTCGTCTCGTCTCCGTCAA
VvGPI	XM_002282738.3	GPI-R	TGGCATCTCTTCGTGTCACTCA
VvFBA	XM_002283345.3	FBA-F	GCTTATTGCCAATGCTGCCTACA
VvFBA	XM_002283345.3	FBA-R	TGTGCCCTTGTCTACTTTGATTCC
VvGAPDH	XM_002263109.3	GAPDH-F	GTCAACGATCCATTCATCACCACT
VvGAPDH	XM_002263109.3	GAPDH-R	AGCAGCCTTGTCCTTGTCAGT
VvPGK	XM_019221715.1	PGK-F	GCCTATGGTATTCTTGATGGTTGGAT
VvPGK	XM_019221715.1	PGK-R	TTTCGCAGTTGCTTTGAGTTAGTCT
VvPGMM	XM_019219069.1	PGMM-F	GAGAAGCATCAGCGACTATCAG
VvPGMM	XM_019219069.1	PGMM-R	CCGTGTCCACAACCAATTCAT
Vvenolase	XM_002267055.2	enolase-F	TGTCACTTGCCGTGTGTAAAGC
Vvenolase	XM_002267055.2	enolase-R	AGCCACCTTCATCACCAACATTG
VvPK	XM_019221576.1	PK-F	ATCACTCTCACTGTCTTGTCTTGT
VvPK	XM_019221576.1	PK-R	AATGTCTTCCTTGTCCTTCTCTGT
VvActin	AB073011.1	Actin-F	GATTCTGGTGATGGTGTGAGT
VvActin	AB073011.1	Actin-R	GACAATTTCCCGTTCAGCAGT

	出峰时间 Peak time (min)	校准曲线 Calibration curve	相关系数R² Correlation coefficient R²	回收率 Recovery rate (%)	相对标准偏差 Relative standard deviation
蔗糖Sucrose	16.493	Y=1.73e+5X-2.47e+2	0.999 1	91.25	0.013
葡萄糖Glucose	19.351	Y=1.31e+5X+5.41e+1	0.994 8	92.14	0.020
果糖Fructose	24.688	Y=1.72e+5X+1.49e+3	0.999 6	98.00	0.009

	出峰时间 Peak time (min)	校准曲线 Calibration curve	相关系数R² Correlation coefficient R²	回收率 Recovery rate (%)	相对标准偏差 Relative standard deviation
蔗糖Sucrose	16.493	Y=1.73e+5X-2.47e+2	0.999 1	91.25	0.013
葡萄糖Glucose	19.351	Y=1.31e+5X+5.41e+1	0.994 8	92.14	0.020
果糖Fructose	24.688	Y=1.72e+5X+1.49e+3	0.999 6	98.00	0.009