Comparative Study on Pollen Viability and Stigma Availability  (Prunus dulcis)

doi:10.6048/j.issn.1001-4330.2020.08.009

Xinjiang Agricultural Sciences ›› 2020, Vol. 57 ›› Issue (8): 1450-1456.DOI: 10.6048/j.issn.1001-4330.2020.08.009

• Animal Husbandry Veterinarian·Forestry • Previous Articles Next Articles

Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)

ZHANG Xiangfei¹, YUE Wanwan¹, LIU Quanxin¹, ZENG Bin¹, HAO Qing², Abudoukayoumu Ayimaiti¹

1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
2. Institute of Horticulture Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China

Received:2019-12-02 Published:2020-09-01
Correspondence author: ZENG Bin (1970-), male, Hubei native, associate professor, doctor, master's supervisor, research direction is fruit tree germplasm resources and fruit tree cultivation physiology, (E-mail) zbxnd@163.com HAO Qing (1969-), male, Aksu, Xinjiang, researcher, master's supervisor, research direction is Xinjiang characteristic fruit tree cultivation, (E-mail) haoqingxj@sohu.com
Supported by:
Supported by the Natural Science Foundation Project of Xinjiang Uygur Autonomous Region “Cloning and Functional Analysis of SSK1, a Key Factor of Self-incompatibility and Non-S Gene in Almonds (Prunus dulcis) in Xinjiang” (2019D01A56); the National Natural Science Foundation of China “Study on Molecular Mechanism of Self-incompatibility Ubiquitin Characteristics of Xinjiang Wild Almonds (Prunus dulcis) Based on S-nuclease” (31660557); Key Discipline Project of Horticulture in Xinjiang Uygur Autonomous Region “Development and Utilization of Characteristic Fruit Tree Germ Resources and Breeding and Popularization of Excellent Varieties in Xinjiang” (2016-10758-3)

几种不同巴旦木花粉生活力及柱头可授性比较分析

张翔飞¹, 岳婉婉¹, 刘泉鑫¹, 曾斌¹, 郝庆², 阿布都卡尤木·阿依麦提¹

1.新疆农业大学林学与园艺学院, 乌鲁木齐 830052;
2.新疆农业科学院园艺作物研究所,乌鲁木齐 830091

通讯作者: 曾斌(1970-),男,湖北人,副教授,博士,硕士生导师,研究方向为果树种质资源及果树栽培生理,(E-mail)zbxnd@163.com 郝庆(1969-),男,新疆阿克苏人,研究员,硕士生导师,研究方向为新疆特色果树栽培,(E-mail)haoqingxj@sohu.com
作者简介:张翔飞(1994-),男,河南禹州人,硕士研究生,研究方向为园艺作物栽培,(E-mail)1185251971@qq.com
基金资助:
新疆维吾尔自治区自然科学基金“新疆巴旦木自交不亲和非S基因关键因子SSK1的克隆及其功能分析研究”(2019D01A56);国家自然科学基金“基于S-核酸酶的新疆野生扁桃自交不亲和性泛素化特性的分子机制研究”(31660557);新疆维吾尔自治区园艺学重点学科“新疆特色果树种质资源开发利用与优良品种选育推广”(2016-10758-3)

Abstract

Abstract: 【Objective】 To provide a reference and theoretical support for the practice of artificial pollination and related research of almonds in the hope of providing a preliminary theoretical basis for the further smooth study of affinity pollination.【Method】 Six almonds varieties at home and abroad were used as experimental materials, I₂-KI staining method was used to determine the changes of pollen activity of almonds varieties on different anthesis days, and benzidine hydrogen peroxide method was used to determine the stigmatization of almonds varieties.【Result】 The pollen vigor determined by the two methods was the largest and the smallest was Make; the pistil length of Shitou, Jianzuihuang and Yingzui reached the maximum and strong acceptability on the second day after anthesis, and the pistil length of Mission, Butter and Make reached the maximum and had strong acceptability on the 3rd day after anthesis , and the pistil length of Shitou, Jianzuihuang and Yingzui reached the maximum and strong acceptability on the 3rd day after anthesis, and the pistil length of Mission, Butter and Make reached the maximum and strong acceptability on the 3rd day after anthesis.【Conclusion】 The pollen collected on the second day after anthesis and the Shitou on the 2nd day after anthesis, Jianzuihuan, Yingzui and the Mission, Butter and Make stigmas on the 3rd day were artificially powdered, which provided the theoretical basis for the breeding of almond varieties.

Key words: almond; pollen vitality; stigma receptivity

摘要： 【目的】 研究巴旦木花粉生活力,比较分析柱头可授性,为巴旦木人工授粉及相关研究和巴旦木良种选育提供理论依据。【方法】 以6个国内外巴旦木品种为材料,采用I₂-KI染色法、离体萌发法法测定不同开花天数的巴旦木花粉活力的变化;用联苯胺-过氧化氢法测定巴旦木各品种的柱头可授性。【结果】 2种方法测定的花粉活力最大的为米星,最小的为麻壳;石头、尖嘴黄、鹰嘴的雌蕊在花开放后第2 d花柱长度达到最大且具有较强的可授性,米星、布特、麻壳的雌蕊在花开放第3 d花柱长度达到最大且具有较强的可授性。【结论】 采集花后第2 d的花粉对花后第2 d的石头、尖嘴黄、鹰嘴和第3 d的米星、布特、麻壳柱头进行人工授粉。

关键词: 巴旦木, 花粉活力, 柱头可授性

CLC Number:

S644.9

ZHANG Xiangfei, YUE Wanwan, LIU Quanxin, ZENG Bin, HAO Qing, Abudoukayoumu Ayimaiti. Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)[J]. Xinjiang Agricultural Sciences, 2020, 57(8): 1450-1456.

张翔飞, 岳婉婉, 刘泉鑫, 曾斌, 郝庆, 阿布都卡尤木·阿依麦提. 几种不同巴旦木花粉生活力及柱头可授性比较分析[J]. 新疆农业科学, 2020, 57(8): 1450-1456.

References

[1] Kester D.E., Kader A.A., and Cunningham S. Encyclopedia of Food Sciences and Nutrition (Second Edition) [M] . Copyright: Elsevier Science Ltd., Netherlands, 2003:150-155.
[2] 李疆, 胡芳名, 李文胜, 等.扁桃的栽培及研究概况[J].果树学报, 2002,19(5): 346-350.
LI Jiang, HU Fangming, LI Wensheng, et al. Advances of almond growing and research [J]. Journal of Fruit Science, 2002,19(5): 346-350.
[3] 张文越, 马玉敏, 孙海伟, 等.扁桃花粉形态观察及人工授粉试验[J].落叶果树, 2000,(6):17-18.
ZHANG Wenyue, MA Yumin, SUN Haiwei, et al. Observation on Pollen Morphology and artificial pollination of almond [J]. Deciduous Fruit Tree, 2000,(6):17-18.
[4] 乔进春, 朱梅玲, 杨敏生, 等. 扁桃的开花结实特性[J].果树学报, 2002, (3):167-170.
QIAO Jinchun, ZHU Meiling, YANG Minshen, et al. Flowering and fruiting characteristics of almond [J]. Journal of Fruit Science, 2002,(3):167-170.
[5] 刘梦婕, 李鹏, 宋恬, 等. 扁桃花粉生物学特性观测[J].新疆农业大学学报, 2018, 41(2):93-101.
LIU Mengjie, LI Peng, SONG Tian, et al. Observation of Biological Characteristics of Amygdalus communis L. [J]. Journal of Xinjiang Agricultural University, 2018,41(2):93-101.
[6] 徐叶挺, 龚鹏, 杨波, 等. 不同贮藏条件下引进扁桃花粉活力变化[J].新疆农业科学, 2011, 48(1):60-63.
XU Yeting, GONG Peng, YANG Bo, et al. Change of Vitality of Introduced Almond Pollen under Different Storage Conditions [J]. Xinjiang Agricultural Sciences, 2011, 48(1):60-63.
[7] 王琳, 梁峰, 郭莎, 等. 不同保存方式对扁桃花粉保存效果的影响[J].中国果树, 2014, (5):33-36.
WANG Lin, LIANG Feng, GUO Sha, et al. Effects of different preservation methods on pollen preservation of almond [J]. China Fruits, 2014,(5):33-36.
[8] 孙颖, 王阿香, 陈士惠, 等. 野生侧金盏花的花粉活力与柱头可授性[J].草业科学, 2014, 31(6):1045-1051.
SUN Ying, WANG Axiang, CHEN Shihui, et al. Research on pollen viability and stigma receptivity of Adonis amurensis [J]. Pratacultural Science, 2014, 31(6):1045-1051.
[9] 王士泉. 四川牡丹花粉生活力测定方法的比较研究[J].热带作物学报, 2020,41(1): 57-62.
WANG Shiquan. Comparative Study on Determination Methods of Pollen Viability in Paeonia decomposita [J]. Chinese Journal of Tropical Crops, 2020,41(1): 57-62.
[10] 罗克明, 马红叶, 杨勇胜, 等. 14个车厘子品种的花粉活力及柱头的可授性研究[J].耕作与栽培, 2019, (2):29-31.
LUO Keming, MA Hongye, YANG Yongshen, et al. Pollen Viability and Stigma Receptivity of 14 Cheli Cultivars [J]. Tillage and Cultivation, 2019,(2):29-31.
[11] 何小弟, 赵正兰, 周魁, 等. 17种梅(桃)属观赏树木花粉生活力的比较[J].南京林业大学学报(自然科学版),2005,(2):29-32.
HE Xiaodi, ZHAO Zhenglan, ZHOU Kui, et al. Comparison of pollen viability of 17 species of ornamental trees of the genus prunes [J]. Journal of Nanjing Forestry University(Natural Science Ed.),2005,(2): 29-32.
[12] 田嘉, 李疆, 高启明, 等. 新疆扁桃花粉萌发及花柱离体培养试验研究[J].新疆农业大学学报, 2010, 33(1):11-14.
TIAN Jia, LI Jiang, GAO Qiming, et al. Experimental Study of Pollen Germination and Style in Vitro Culture of Xinjiang Almond Cultivars [J]. Journal of Xinjiang Agricultural University, 2010, 33(1):11-14.
[13] 金亚征,忻龙祚,王建民,等.培养基成分对仁用杏花粉离体萌发的影响[J].经济林研究, 2014, 32(1):101-106.
JIN Yazheng, XIN Longzhu, WANG Jianmin, et al. Effects of medium components on Pollen Germination of almond apricot in vitro [J]. Economic Forest Research, 2014, 32(1):101-106.
[14] 赵晖, 马海霞, 李胜, 等. 扁桃雌蕊形态及其保水能力研究[J]. 甘肃农业大学学报, 2004, (3):285-289.
ZHAO Hui, MA Haixia, LI Sheng, et al. A study on morphology and ability to maintain moisture of pistil of almond [J]. Journal of Gansu Agricultural University, 2004,(3):285-289.
[15] 陈梦华, 赵丹, 靳丽鑫, 等. 麻核桃花粉生活力与柱头可授性对坐果的影响[J].北方园艺, 2016, (3):28-30.
CHEN Menghua, ZHAO Dan, JIN Lixin, et al. Effect of Pollen Viability and Stigma Receptivity of Juglans hopeiensis Hu to Fruit Setting [J]. Northern Horticulture, 2016,(3):28-30.
[16] 李百云, 谢志强, 魏天军. 宁夏枣的花粉活力和柱头可授性[J].江苏农业科学,2015,43(5):171-172.
LI Baiyun, XIE Zhiqiang, WEI Tianjun. Pollen viability and stigma confer ability of jujube in Ningxia [J].Jiangsu Agricultural Sciences, 2015, 43(5):171-172.
[17] 郭媛, 邵有全, 郭宝贝, 等.梨花粉和柱头发育与温度关系研究[J].中国生态农业学报,2014,22(12):1446-1452.
GUO Yuan, SHAO Youquan, GUO Baobei, et al. Studies on the relationship between pollen and stigma development and temperature in pear [J]. Chinese Journal of Eco-Agriculture, 2014, 22(12):1446-1452.
[18] 李勇军, 尹桂芳, 马继琼, 等. 魔芋花粉生活力检测方法的比较研究[J].西南农业学报, 2012, 25(5):1790-1793,1959.
LI Yongjun, YIN Guifang, MA Jiqiong, et al. Comparison of Detection Methods of Pollen Viability in Konjac [J]. Southwest China Journal of Agricultural Sciences, 2012, 25(5):1790-1793,1959.
[19] 涂清芳, 贾雪晴, 姜自红, 等. 4种方法检测8个菊花品种花粉活力的比较[J]. 江苏农业科学, 2019, 47(2):109-111.
TU Qingfang, JIA Xueqing, JIANG Zihong, et al. Comparison of four methods for detecting pollen vigor of eight Dendranthema morifolium varieties [J]. Jiangsu Agricultural Sciences, 2019, 47(2):109-111.
[20] 王伟伟, 姚玲, 何开跃, 等. 不同柳树花粉离体培养及活力测定方法比较研究[J]. 江苏农业科学, 2018, 45(4):1-7.
WANG Weiwei, YAO Ling, HE Kaiyue, et al. Comparative study on in vitro pollen germination and viability evaluation of different willow species [J]. Jiangsu Agricultural Sciences, 2018, 45(4):1-7.
[21] 左丹丹, 明军, 刘春, 等. 植物花粉生活力检测技术进展[J]. 安徽农业科学, 2007, 35(16):4742-4745.
ZUO Dandan. MING Jun, LIU Chun, et al. Advance in Technique of Plant Pollen Viability [J]. Journal of Anhui Agricultural Sciences, 2007,35(16):4742-4745.
[22] 朱梅玲, 乔进春. 扁桃花粉数量及花粉质量的检测[J]. 河北果树, 2004, (6):8-11.
ZHU Meiling, QIAO Jinxi. Detection of pollen quantity and pollen quality of almond [J]. Hebei Fruits, 2004, (6):8-11.

Comparative Study on Pollen Viability and Stigma Availability (Prunus dulcis)

几种不同巴旦木花粉生活力及柱头可授性比较分析

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

[1]	SONG Danbo, ZHANG Guoxin, WANG Quan, LIU Baojun, HUANG Tao, HAN Hongwei, BAI Jianyu, GUO Qingyuan. Study on the biological characteristics of the pathogen of bacterial perforation of alternaria alternata in Xinjiang and the screening of laboratory agents [J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1515-1522.
[2]	TANG Xixi, Mubarek Ayup, XU Panyun, YU Qiuhong, GUO Chunmiao, ZHANG Ping, GONG Peng. Response of root anatomical structure of different rootstock resources of almond to drought stress [J]. Xinjiang Agricultural Sciences, 2023, 60(4): 897-907.
[3]	XU Yeting, ZHANG Xiaoli, ZHUANG Hongmei, DONG Wenxuan, GONG Peng. Determination of Dangshan Pear Pollen Vitality Using Near -Infrared Spectroscopy [J]. Xinjiang Agricultural Sciences, 2022, 59(3): 617-624.
[4]	XIE Hui, HAN Shouan, WANG Min, ZHANG Wen. Effect of Trees from on Grain Yield and Photosynthetic Characteristics at Different Growth Stage of Wheat under Almond Wheat Intercropping [J]. Xinjiang Agricultural Sciences, 2021, 58(9): 1610-1623.
[5]	ZHANG Li, BIAN Bo, Tuluhong Tuerdi, WANG Xuenong. Experiment and Optimization Analysis of Air Suction Type for Almonds Shell Kernel Sorting Device [J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1540-1546.
[6]	YANG Bo, GUO Chunmiao, Mubareke Ayoupu, GONG Peng. Study on Genetic Diversity and Probability Grading of Quantitative Traits in Stones of Native Almond Germplasm Resources in Xinjiang [J]. Xinjiang Agricultural Sciences, 2021, 58(7): 1297-1305.
[7]	BIAN Bo, Tuluhong Tuerdi, ZHANG Li, WANG Xuenong. Research and Experiment on Material and Aerodynamic Characteristics of Almonds Shell and Kernel [J]. Xinjiang Agricultural Sciences, 2021, 58(5): 893-902.
[8]	YANG Bo, GUO Chunmiao, Mubareke Ayoupu, XIAO Li, XU Juan, GONG Peng, XU Yeting. Researches between Lignification of Almond Fruit Endocarp and the Activities of Related Enzyme [J]. Xinjiang Agricultural Sciences, 2021, 58(1): 23-29.
[9]	ZHANG Wen, ZHOU Hao, LIU Cuirong, ZHOU Yumei, XIE Hui. Effects of Different Tree Shapes on Regional Light Environment in Almond-Winter Wheat Intercropping Areas [J]. Xinjiang Agricultural Sciences, 2020, 57(9): 1639-1649.
[10]	Abudoukayoumu Ayimaiti, FAN Dingyu, HAO Qing, ZENG Bin, ZHANG Xiangfei, YU Zhenfan, TANG Yilian. Study on Pollen Germination Characteristics of Jujube and its Response to Gibberellin [J]. Xinjiang Agricultural Sciences, 2020, 57(2): 283-291.
[11]	WANG Min, LI Jiang, LI Peng, TIAN Jia, LUO Shuping. Cloning and Prokaryotic Expression of Almond AcDHN1 Gene [J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2221-2229.
[12]	SUN Zezhao, ZHANG Renfu, WANG Wei, LIU Haiyang, YAO Ju. Effects of Main Pests and Predatory Natural Enemies on Cotton under Almond-cotton Interplanting [J]. Xinjiang Agricultural Sciences, 2020, 57(11): 2044-2049.
[13]	LI Shao-ze, ZHANG Zhi-gang, CHENG Ping, YANG Lu, LI Hong, REN Ya-qin, WANG Dong-liang, WANG Zi-long. The Change of Endogenous Hormone of Apricot Tree in Flowering and Setting Period [J]. Xinjiang Agricultural Sciences, 2019, 56(2): 258-266.
[14]	ZHANG Qi, LI Peng, TIAN Jia, LI Jiang. Study on Photosynthetic and Chlorophyll Fluorescence Characteristics of Transgenic Tobacco with AcCBF1 Gene [J]. Xinjiang Agricultural Sciences, 2019, 56(2): 267-277.
[15]	LIU Yan-bin, Tuluhong Tuerdi, YANG Hui-min, SAN Yun-long, WANG Xue-nong. Effects of Thickness and Moisture Content on Mechanical Properties of Almond Shell Breaking [J]. Xinjiang Agricultural Sciences, 2018, 55(7): 1293-1304.