Abstract: [Objective] To evaluate the potential of camel-derived antibody cAbBCII10 as a non-antibody affinity transfer skeleton, the FSHR binding peptide was grafted to the antigen-binding region of cAbBCII10 using affinity transfer to rapidly obtain anti-FSHR antibody.[Method]The FSH binding motif FSH 33-53 coding sequence was inserted into the CDR1 and CDR3 regions of the nanobody cAbBCII10, respectively, and named VHH-hFSH1 and VHH-hFSH3.These DNA sequences were cloned into pET22b vector and transformed into E.coli BL21 (DE3), and purified single-domain antibody was obtained by IPTG induction and Ni-affinity affinity chromatography.[Result]Finally binding capacity and specificity of purified cAbBCII10, VHH-hFSH1 and VHH-hFSH3 with FSHR were identified by ELISA.The VHH-hFSH1, VHH-hFSH3 and cAbBCII10 proteins obtained by the framework transplantation were expressed in the intercellular space of bacteria.ELISA experiments showed that the VHH-hFSH3 obtained by grafting the FSHR binding peptide FSH33-53 to the CDR3 of cAbBCII10 had a specific binding to FSHR activity.[Conclusion]CAbBCII10 can be used as a graft framework, FSH and FSHR binding peptide grafted into the CDR1 and CDR3 regions of cAbBCII10 can obtain higher affinity anti-FSHR antibody.