Abstract: [Objective] In order to study the adaptability of blueberries to potted soil environment in Xinjiang, the humus soil in the spruce forest of Tianshan Mountain was mixed with garden soil in proportion and was supplemented with decomposed sheep manure and humic acid to configure the composite substrate.The growth and physiological indexes of different blueberry varieties in the substrate were analyzed in order to provide the basic theoretical basis for the promotion and comprehensive utilization of blueberry in Xinjiang.[Method]Under the greenhouse condition, four blueberry varieties were used as experimental materials, such as Bluecrop, Northland, Darrow and Duke.Two-way ANOVA experiment was used to design the four blueberry varieties with four different combinations of the medium.The growth of seedlings and the photosynthetic indexes of the leaves of blueberries in the substrate were observed.The effects of the substrate on the growth of blueberries were analyzed by principal component analysis (PCA).The correlation between the substrate and the growth of blueberries was analyzed using Pearson correlation.[Result]The pH of the composite substrate did not meet the conditions of blueberry growth.The pH of the substrate and irrigation water was adjusted with acetic acid.The physical and chemical indexes of the substrate could meet the requirements of the blueberry growth environment.The effect of the substrate and varieties on the growth and photosynthetic indexes of blueberries were all significant.The effect of the substrate was greater than that of cultivars.The comprehensive impact of the growth of the two blueberry varieties such as Northland and Darrow in the composite substrate of humus soil and garden soil by 2∶1 to 3∶1 was higher.The growth of the two blueberry varieties of Bluecrop and Duke was correlated with the EC of the substrates.Its difference was significant.[Conclusion]The results showed that the surface humus and soil ratio of Tianshan spruce forest were mixed as the composite substrate in the range of 2∶1 to 3∶1, with supplementing decomposed sheep manure and humic acid.Acetic acid composite substrate and irrigation water to pH 4.5 can replace peat for the two potted blueberry varieties such as Northland and Darrow.