不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响

doi:10.6048/j.issn.1001-4330.2018.02.021

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (2): 371-378.DOI: 10.6048/j.issn.1001-4330.2018.02.021

不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响

雒诚龙¹,邵伟^1,2,任万平¹,赵艳坤¹,王东海¹,余雄²

1.新疆农业大学动物科学学院,乌鲁木齐 830052;
2.新疆肉乳用草食动物营养重点实验室,乌鲁木齐 830052

收稿日期:2018-01-31 出版日期:2018-02-20 发布日期:2018-06-30
通信作者: 余雄(1958- ),男,四川邻水人,教授,博士生导师,研究方向为反刍动物营养,(E-mail)8769651@163.com
作者简介:雒诚龙(1993- ),男,甘肃甘谷人,硕士研究生,研究方向为细胞与分子生物学,(E-mail)422746985@qq.com
基金资助:
现代农业产业技术体系建设专项资金资助(CARS-37);新疆维吾尔自治区重点实验室开放课题(2015KL018);中国博士后基金委资助项目;国家自然基金项目(31560645)

The Impact of Various Biotin Levels on Adipogenetic Relevant Gene Transcription and Expression of 3T3-L1 Adipocytes

LUO Cheng-long¹,SHAO Wei^1,2,REN Wan-ping¹,ZHAO Yan-kun¹,WANG Dong-hai¹,YU Xiong²

1.Collage of Animal Science,Xinjiang Agricultural University,Urumqi 830052, China;
2. Xinjiang Key Laboratory of Meat and Milk Production Herbivore Nutrition,Urumqi 830052, China

Received:2018-01-31 Online:2018-02-20 Published:2018-06-30
Correspondence author: YU Xiong (1958- ), male, native place: Lingshui, Sichuan. Professor, research field: Ruminant nutrition. (E-mail) 8769651@163.com

摘要/Abstract

摘要： 【目的】诱导3T3-L1细胞分化为3T3-L1脂肪细胞,研究不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响。【方法】利用三联诱导法将3T3-L1细胞经诱导分化为3T3-L1脂肪细胞。当3T3-L1脂肪细胞密集后分别采用0(对照组)、0.2、0.5、1 μmol/L生物素处理,分别在12 h、24 h与48 h时检测细胞上清液中PK mRNA、GLUT-4 mRNA、FAS mRNA及ACC1 mRNA相对表达量。【结果】在试验12 h时:各试验组GLUT-4、PK、ACC1 mRNA相对表达量均极显著(P＜0.01)高于对照组,1 μmol/L组与0.5 μmol/L组FAS mRNA相对表达量极显著(P＜0.01)高于对照组与0.2 μmol/L组;在试验24 h时:1 μmol/L组GLUT-4 mRNA与PK mRNA相对表达量显著(P＜0.05)高于对照组,1 μmol/L组ACC1 mRNA相对表达量极显著(P＜0.01)高于对照组,0.2 μmol/L组与0.5 μmol/L组ACC1 mRNA相对表达量显著(P＜0.05)高于对照组;在试验48 h时:1 μmol/L组GLUT-4 mRNA相对表达量显著(P＜0.05)高于对照组,1 μmol/L 组PK mRNA相对表达量极显著(P＜0.01)高于对照组,0.5 μmol/L组FAS mRNA相对表达量极显著(P＜0.05)高于1 μmol/L组,1 μmol/L组FAS mRNA相对表达量显著(P＜0.05)高于对照组,1 μmol/L组ACC1 mRNA相对表达量极显著(P＜0.01)高于其它组。【结论】生物素的添加可以提升脂肪细胞GLUT-4、PK、FAS与ACC1 mRNA相对表达量,且当以1 μmol/L浓度作用时对GLUT-4、PK与ACC1提升效果最佳,以0.5 μmol/L浓度作用时对FAS提升效果最佳。

关键词: 生物素; 3T3-L1脂肪细胞; 脂合成

Abstract: 【Objective】 In this study, the purpose is to induce 3T3-L1 cells to differentiate into 3T3-L1 adipocytes and to study the effects of various biotin levels on the transcription and expression of adipose synthase related genes in 3T3-L1 adipocytes.【Method】3T3-L1 cells were induced to differentiate into 3T3-L1 adipocytes by triplex induction. When 3T3-L1 adipocytes were dense, they were treated by 0 μmol/L biotin (control group), 0.2, 0.5 and 1 μmol/L The relative expression of competitive PKmRNA, GLUT-4 mRNA, FAS mRNA and ACC1 mRNA in cell supernatant was detected at 12h, 24h and 48h, respectively.【Result】Results showed that when the test time was 12 h: GLUT-4 mRNA, PK mRNA and ACC1 mRNA relative expression quantity of experimental groups was significantly (P＜0.01) higher than that of control group, FAS mRNA relative expression quantities of 0.5 μmol/L group and 1 μmol/L group were significantly (P＜0.01) higher than that of control group. When the test time was 24 h: GLUT-4 mRNA and PK mRNA relative expression quantity of 1 μmol/L group was significantly (P＜0.05) higher than that of control group, ACC1 mRNA relative expression quantity of 1 μmol/L group was extremely significantly higher (P＜0.01) than that of control group,ACC1 mRNA relative expression quantity of 0.2 μmol/L group and 0.5 μmol/L group was significantly higher (P＜0.05) than that of control group. When the test time was 48 h, GLUT-4 mRNA relative expression quantity of 1 μmol/L group was significantly (P＜0.05) lower than that of control group, PK mRNA relative expression quantity of 1 μmol/L group was extremely significantly (P＜0.01) higher than that of control group, FAS mRNA relative expression quantity of 0.5 μmol/L group was significantly (P＜0.05) higher than that of 1 μmol/L group, FAS mRNA relative expression quantity of 1 μmol/L group was significantly (P＜0.05) higher than that of control group, ACC1 mRNA relative expression quantity of 1 μmol/L group was extremely significantly (P＜0.01) higher than that of other groups.【Conclusion】The addition of biotin can inhibit the adipogenesis through promoting the relative expression of GLUT-4, PK, FAS and ACC1 mRNA in adipocytes. Moreover, when the concentration is equal to 1 μmol/L, the effect is optimal to GLUT-4, PK and ACC1 mRNA, and when the concentration is equal to 0.5 μmol/L, the effect is optimal to FAS mRNA.

Key words: biotin; 3T3-L1 adipocytes; adipogenesis

中图分类号:

Q253
S188

雒诚龙,邵伟,任万平,赵艳坤,王东海,余雄. 不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响[J]. 新疆农业科学, 2018, 55(2): 371-378.

LUO Cheng-long,SHAO Wei,REN Wan-ping,ZHAO Yan-kun,WANG Dong-hai,YU Xiong. The Impact of Various Biotin Levels on Adipogenetic Relevant Gene Transcription and Expression of 3T3-L1 Adipocytes[J]. Xinjiang Agricultural Sciences, 2018, 55(2): 371-378.

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不同生物素水平对3T3-L1脂肪细胞脂肪合成相关基因转录表达的影响

The Impact of Various Biotin Levels on Adipogenetic Relevant Gene Transcription and Expression of 3T3-L1 Adipocytes

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