新疆地方梨品种S基因型的鉴定

doi:10.6048/j.issn.1001-4330.2018.02.006

新疆农业科学 ›› 2018, Vol. 55 ›› Issue (2): 246-252.DOI: 10.6048/j.issn.1001-4330.2018.02.006

新疆地方梨品种S基因型的鉴定

张校立,徐叶挺,艾沙江·买买提,许娟,邓莉,王继勋

新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐 830091

收稿日期:2018-01-16 出版日期:2018-02-20 发布日期:2018-06-30
通信作者: 王继勋(1965-),男,安徽砀山人,研究员,研究方向为果树资源与育种,(E-mail)924101849@qq.com
作者简介:张校立(1981-),男,河南上蔡人,助理研究员,硕士,研究方向为梨遗传育种与栽培,(E-mail)Zhangxiaoli2002@126.com
基金资助:
自治区公益性科研院所基本科研业务经费资助项目(KYGY2016118);新疆农业科学院青年科技基金项目(xjnkq-2015024)

Identification of S-genotypes of Native Pear Cultivars in Xinjiang

ZHANG Xiao-li, XU Ye-ting, Aishajiang Maimaiti, XU Juan, DENG Li, WANG Ji-xun

Research Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences / Xinjiang Fruit Science Observation and Experiment Station, Ministry of Agriculture of China, Urumqi 830091,China

Received:2018-01-16 Online:2018-02-20 Published:2018-06-30
Correspondence author: WANG Ji-xun(1965-),male,Researcher,Research direction:Fruit tree resources and breeding, ,(E-mail)924101849@qq.com

摘要/Abstract

摘要： 【目的】鉴定与研究9个新疆地方梨品种S基因型,为库尔勒香梨授粉品种的筛选及其遗传改良提供理论依据。【方法】设计特异性引物对9 个新疆地方梨品种的基因组DNA 进行PCR扩增,片段回收、克隆、测序,使用生物信息学软件对各序列进行分析。【结果】褐色句句梨、轮台句句梨、霍城冬黄梨、八月梨、库尔勒黄酸梨、句句梨、奎克阿木特1号等7个品种的S基因型分别是:S28S4、S28S4、S28S1、S28S4、S22S35、S28S4、S36S34S22;可特阿木特和棋盘梨各鉴定出一条基因,S基因型分别是 S8Sx和 S28Sx。【结论】确定了7个新疆地方梨品种S基因型,可特阿木特和棋盘梨中各有 1条 S 基因没有被鉴别出。丰富了我国梨资源的S基因型信息,为库尔勒香梨的丰产栽培和遗传改良提供了依据。

关键词: 新疆地方梨品种; S基因型; DNA 序列分析

Abstract: 【Objective】 To provide theoretical basis for the selection of pollinated varieties and genetic improvement of Korla pear by classifying and studying the S genotypes of 9 native pear varieties in Xinjiang.【Method】A specific primer was designed to amplify the genomic DNA of 9 native pear varieties in Xinjiang, including PCR amplification, fragment recovery, cloning, sequencing, and the analysis of each sequence by bioinformatics software.【Result】The S-genotypes of the seven cultivars were identified as follows: Hesejujuli S28S4, Luntaijujuli S28S4, Hesejujuli:S28S4,Luntaijujuli:S28S4,Huochengdonghuangli:S28S1,Bayueli:S28S4,Kuerlehuangsuanli:S22S35,Jujuli:S28S4,Kuikeamute1hao:S36S34S22.One gene was identified as S8Sx and another gene as S28Sx in Keteamute Pear and Qipanli Pear, respectively.【Conclusion】It has enriched the s-genotypes information of pear cultivars in China and provided a certain guidance gist for high yield cultivation and genetic improvement of Korla pear.

Key words: native pear cultivars in Xinjiang; S-genotype; DNA sequence analyses

中图分类号:

S188
S661.2

张校立,徐叶挺,艾沙江·买买提,许娟,邓莉,王继勋. 新疆地方梨品种S基因型的鉴定[J]. 新疆农业科学, 2018, 55(2): 246-252.

ZHANG Xiao-li, XU Ye-ting, Aishajiang Maimaiti, XU Juan, DENG Li, WANG Ji-xun. Identification of S-genotypes of Native Pear Cultivars in Xinjiang[J]. Xinjiang Agricultural Sciences, 2018, 55(2): 246-252.

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新疆地方梨品种S基因型的鉴定

Identification of S-genotypes of Native Pear Cultivars in Xinjiang

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