Abstract: Objective]In order to provide technical support for improving production and efficiency of Agaricus bisporus,the fermentation technology of simple oxygen ventilation of Agaricus bisporus substrate were developed to improve the technical level of fermentation of the small scale farming cultivation.[Method]De-vices of simple oxygen ventilation and double plastic film covering were used to treat the Agaricus bisporus sub-strate.[Result]The fermentation technology of simple oxygen ventilation could reduce the area of anaerobic fermentation,maintain the temperature and humidity of substrate pile,promote the growth of actinomycetes, ensure the homogeneity and stability of fermentation and improve the effect of fermentation,shorten the fer-mentation time of 3 to 5 days and increase the area of actinomycetes of 6 1;.[Conclusion]Simple ventilation oxygen fermentation technology has the advantages of simple operation,low cost,which effectively improved the fermentation quality and efficiency of the double spore mushroom culture medium and it is so suitable for the small scale farming cultivation of Agaricus bisporus that it must have a good application potential.