Abstract: Objective]Iron deficient chlorosis is an important nutritional disorder of Korla Fragrant Pear (Pyrus Bretschneideri Rehd)grown in calcareous soil in Southern Xinjiang,China.Infusion of iron fertilizer treatment method is expected to become an effective means for treatment of chlorosis of fruit trees.[Method]With the orange chelate generated by orthophenanthroline and ferrous sulfate as tracer material,a simulation test on the transport velocity and concentration distribution characteristics of iron on each part of Pyrus betulifo-lia Bunge after being instilled with iron was performed in the study.[Result]The result showed that the con-tent of iron of test tree with red iron liquid was much higher than the contrast test tree.Active iron and total i-ron contents in treatments were much higher than those in the control (7.5;-435.1;)and (10.2;-341 .9;),respectively.In trunk infusion experiment,Fe transportation speed to the canopy in the trunk was much greater than that downward in the root (49 .00;).Dilution effect of ferrite concentration was observed in the trunk infusion as well as in the root injection.[Conclusion]In the trunk or root infusion,the phenome-non of iron inactivation was observed.Measuring concentration of iron revealed that the trunk infusion was more effective in iron transport and anti-inactivation compared with root infusion.It was suggested to apply trunk infusion to correct iron deficiency chlorosis of fruit trees.