高放射土壤中拮抗放线菌菌株的筛选及鉴定

高放射土壤中拮抗放线菌菌株的筛选及鉴定

李阳;李健强;宋素琴;王静;楚敏

新疆农业大学食品科学与药学学院,乌鲁木齐830052;新疆轻工职业技术学院,乌鲁木齐830021;新疆农业科学院微生物应用研究所,乌鲁木齐830091;中国农业大学植物病理学系,种苗健康北京市工程研究中心,北京100193;新疆农业科学院微生物应用研究所,乌鲁木齐,830091;新疆农业大学食品科学与药学学院,乌鲁木齐,830052

收稿日期:2017-01-25 修回日期:2017-01-25 出版日期:2017-01-25 发布日期:2017-01-25

The Screening and Identification of Antagonistic Actinomycetes Strains from the High Radiation Soil

LI Yang;LI Jian-qiang;SONG Su-qin;WANG Jing;CHU Min

Received:2017-01-25 Revised:2017-01-25 Online:2017-01-25 Published:2017-01-25

摘要/Abstract

摘要： [目的]从新疆高放射土壤中分离和筛选出对苹果树腐烂病等有拮抗作用的放线菌,并对其进行鉴定.[方法]采用对峙培养法通过初筛、复筛测定拮抗菌对8种病原菌的拮抗效果,根据筛选出菌株CH10的形态特征、生理生化特性及16S rDNA序列对其进行鉴定.[结果]从70种放线菌中筛选出对苹果树腐烂病等8种新疆作物重要病原菌都具有良好拮抗效果的CH10.[结论]菌株CH10属于链霉菌属,对苹果树腐烂病菌等有良好的拈抗效果,为该病害的生物防治提供研究基础.

Abstract: [Objective] This project aims to isolate and screen 70 actinomycetes strains from the high radiation soil of Xinjiang by antagonistic effect of 8 kinds of pathogenic bacteria such as the Valsamali Miyabe et Yamada.[Method] By the plate confrontation culture method,and through initial screening and repeated screening of antagonistic effect of 8 kinds of pathogenic bacteria,and according to the morphological characteristics of the strains of CH10,the physiological and biochemical characteristics and 16S rDNA sequence went through identification and analysis.[Result] The results showed that 8 kinds of important pathogens of Xinjiang crops,such as the Valsa mali Miyabe et Yamada,were screened out from 70 kinds of actinomycetes,which had good antagonistic effect called CH10.[Conclusion] Strain CH10 belongs to the genus streptomyces and it has good antagonistic effect on the Valsa mali Miyabe et Yamada,which has provided a foundation for the biological control of the disease.

李阳;李健强;宋素琴;王静;楚敏. 高放射土壤中拮抗放线菌菌株的筛选及鉴定[J]. .

LI Yang;LI Jian-qiang;SONG Su-qin;WANG Jing;CHU Min. The Screening and Identification of Antagonistic Actinomycetes Strains from the High Radiation Soil[J]. .